Author/Authors :
Emami، Shiva نويسنده Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University G.C. Tehran, IR Iran , , Nikokar، Iraj نويسنده Laboratory of Microbiology and Immunology of Infectious Diseases, Paramedicine Faculty, Guilan University of Medical Sciences, Guilan, IR Iran , , Ghasemi، Yusuf نويسنده Department of Biotechnology, Laboratory of Microbiology and Immunology of Infectious Diseases, Para Medicine Faculty, Guilan University of Medical Sciences, Rasht, IR Iran , , Ebrahimpour، Monireh نويسنده Department of Biotechnology, Laboratory of Microbiology and Immunology of Infectious Diseases, Para Medicine Faculty, Guilan University of Medical Sciences, Rasht, IR Iran , , Sedigh Ebrahim-Saraie، Hadi نويسنده Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IR Iran , , Araghian، Afshin نويسنده Department of Biotechnology, Laboratory of Microbiology and Immunology of Infectious Diseases, Para Medicine Faculty, Guilan University of Medical Sciences, Rasht, IR Iran , , Faezi، Sobhan نويسنده Department of Bacteriology, Pasteur Institute of Iran, Tehran, IR Iran , , Farahbakhsh، Mojtaba نويسنده Department of Biochemistry, Faculty of Paramedicine, Guilan University of Medical sciences, Rasht, IR Iran Farahbakhsh, Mojtaba , Rajabi، Abdolhalim نويسنده Student Research Committee, Department of Epidemiology , School of Health and Nutrition, Shiraz University of Medical Sciences, Shiraz, Iran ,
Abstract :
Pseudomonas aeruginosa is considered as a major cause of hospital-acquired infections due to its high antibacterial resistance. Biofilm formation is a well-known pathogenic mechanism in P. aeruginosa infections, since sessile bacteria are protected in an extracellular matrix of exopolysaccharide. The expression of polysaccharide synthesis locus (pslA gene) can be important for biofilm formation by P. aeruginosa. The purpose of this research was to evaluate the antibiotic resistance pattern and distribution of the pslA gene among biofilm-producing P. aeruginosa isolates obtained from waste water of Burn Centre in Guilan, Iran. Fifty isolates of P. aeruginosa were obtained from waste water of a burn center. The P. aeruginosa isolates were identified using standard bacteriological procedures. Drug susceptibility test was performed by disk diffusion method for all the isolates against nine antimicrobial agents. Biofilm formation was measured by microtiter plate assay. Polymerase chain reaction (PCR) was used to identify the presence of the pslA gene among the isolates. Biofilm formation was observed in 70% of the P. aeruginosa isolates. The potential formation of biofilm was significantly associated with resistance to gentamicin, imipenem, tobramycin and piperacillin. In addition, the pslA gene only existed in biofilm-producing isolates with a frequency of 42.9% (n = 15). The findings of the present study well demonstrated that the P. aeruginosa biofilm-producing isolates were more resistant to the tested antibiotics. Furthermore, because of wide distribution, it seems that the pslA gene is associated with biofilm formation
