Validation of PCR Assay for Identification of Sarcoptes scabiei var. hominis.
Naz، Shumaila نويسنده Dept. of Zoology, Faculty of Sciences, University of Pir Mehr Ali Shah- Arid Agriculture, Rawalpindi, Pakistan. Naz, Shumaila , Rizvi، Dilwar Abbas نويسنده Institute of Dermatology, Military Hospital of Rawalpindi, Pakistan. Rizvi, Dilwar Abbas , Javaid، Amara نويسنده Institute of Biomedical and Genetic Engineering, Islamabad, Pakistan. Javaid, Amara , Abrar Ismail، Muhammad نويسنده Director (Urban Development), Earthquake Reconstruction and Rehabilitation Authority Prime Minister Secretariat (Public) Islamabad, Abrar Ismail, Muhammad , Chaudhry، Farhana Riaz نويسنده Dept. of Zoology, Faculty of Sciences, University of Pir Mehr Ali Shah- Arid Agriculture, Rawalpindi, Pakistan. Chaudhry, Farhana Riaz
Background: Infestation of the skin by the “itch mite” Sarcoptes scabiei var. hominis results in a contagious skin infection in humans called “sca-bies”. By resolving morphology issues, the present study was designed to be acquainted with itch mite by molecular markers.
Methods: The mite samples were collected from scabies patients by visiting government hospitals of twin City, Pakistan. For successful mo-lecular detection approach, preparation of Sarcoptes mite DNA by com-mercial DNA extraction kit method. Furthermore, two primers i.e. Sarms 15 F/R and 16S D1/D2 were used to amplify target sequence by using PCR. The amplified products were then separated by agarose gel, electrophoresis and analyzed after staining and visualizing in UV transilluminator.
Results: Analysis of PCR product showed one specific band of 178 bp with primer Sarms 15 F/R, while, with primer 16S D1/D2 bands of 460 bp and 600 bp were observed on 2% agarose gel. The appearance of different band of 600 bp revealed that it might be due to heteroplasmy state present in the Pakistani Sarcoptes mites population.
Conclusion: Current study adds validity to the claim that PCR is more accurate, specific and sensitive in the detection of the ectoparasites even in smallest amount.