Author/Authors :
Pournajaf، Abazar نويسنده Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, IR Iran Pournajaf, Abazar , Ardebili، Abdollah نويسنده Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, IR Iran , , Allah-Ghaemi، Ezzat نويسنده Department of Microbiology, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, IR Iran. Allah-Ghaemi, Ezzat , Omidi، Sajad نويسنده Department of Pathobiology, Department of Microbiology, Faculty of Public Health, Tehran University of Medical Sciences. Tehran, IR Iran. Omidi, Sajad , Borhani، Katayoun نويسنده Department of Microbiology, Pasteur Institute of Iran, Tehran, IR Iran , , Khodabandeh، Mahmoud نويسنده Department of pediatric infectious disease, Faculty of Medicine, Tehran University of medical Sciences, Tehran, IR Iran. Khodabandeh, Mahmoud , Davarpanah، Masoumeh نويسنده Department of Microbiology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, IR Iran. Davarpanah, Masoumeh
Abstract :
Background: Infections resulted from multi-resistant Staphylococcus aureus are increasing worldwide. In the present study, a Multiplex-PCR assay for the detection of antibiotic resistance genes among S. aureus clinical isolates and for the concomitant identification of these isolates
was described.
Methods: A total of 127 S. aureus isolates were collected from clinical specimens at three teaching hospitals in Tehran, Iran. Screening for methicillin and mupirocin resistance in staphylococcal isolates was performed by disk diffusion method, according to Clinical and
Laboratory Standards Institute (CLSI) guidelines. The presence of femB, mecA, and iles-2 genes was investigated by multiplex-PCR.
Results: The 62.2% and 7.8% of Staphylococcus isolates were positive for mecA and ileS-2 genes, respectively. The femB fragment was amplified in all S. aureus strains tested. There is a 100% concordance between susceptibility and PCR results for isolates which harbored ileS-2. However, 55.1% of staphylococci were found as MRSA in the phenotypic assay.
Conclusions: The PCR assay described in this study was able detect three genes for identification of S. aureus and its methicillin and mupirocin resistant genotypes concomitantly in a single reaction. Hence, this method can be used on regular basis as a valuable diagnostic and surveillance tool in clinical laboratories.
