Determination of Inhibitory Concentration of Oxytetracycline on Methanogenic Bacteria by in vitro Study
Hashemi، Hassan نويسنده , , Safari، Mahdi نويسنده Department of Statistics, Lorestan University of Medical Sciences, Khorramabad, Iran Safari, Mahdi , Khodabakhshi، Abbas نويسنده Department of Environmental Health Engineering, Shahrekord University of Medical Sciences, Shahrekord, Iran ,
Introduction: Antibiotics have the potential to adversely affect the microbial community. For anaerobic digestion, a sufficient methanogenic population needs to be preserved in the system. The main aim of this study was determination of inhibitory concentration of oxytetracycline on methanogenic bacteria.
Methods: A 120 mL jacketed bioreactor with a 90 mL working volume was inoculated granular sludge from an anaerobic digester, substrate and different concentration of oxytetracycline with 10 days cycles and intermittent mixing. The reactor was operated at 35 ± 2 ° C. The inhibitory effect of antibiotic was evaluated by monitoring biogas production.
Results: Based on the findings from each batch, complete inhibitory concentration of oxy tetracycline was in concentration of 800 mg L-1. Significant relation was seen between inoculated antibiotic concentrations and methane production (r=-0.86).
Conclusion: The addition of antibiotics to the biomass affected the utilization of fatty acids, resulting in unfavorable effects on methanogenesis. Thus, overusing of antibiotics can adverse effects of intestinal flora.