Microbiological Assessment of the Bio filter Matrix Within a Biofiltration system Treating Borehole water in Kwa Zulu-Natal (SouthAfrica)

To assess the microbiological status of a biofiltration system used to treat borehole water, filter matrix samples were analyzed after staining for the presence of active biofilms using confocal laser scanning microscopy (CLSM). CLSM revealed the presence of biofilms on the filter matrix with actively metabolizing microbial cells present. Thereafter, heterotrophs,manganese oxidizing bacteria (MOB) and iron oxidizing bacteria (IOB) present in the biofilms were quantified. For heterotrophs a count of 2.9×107 cfu/g was established using R2A agar while counts for presumptive MOB and IOB were established as 2.4×107 cfu/g and 3.1×107 cfu/g respectively. In addition, a clone library was established using DNAextracted from a pooled filter matrix sample to assess the diversity of bacteria present within the biofilter matrix. A total of 100 randomly selected clones were separated into 14 unique operational taxonomic unit (OTU’s) based upon restriction patterns of amplified partial 16S rRNA genes. Overall, 38% of the clones were assigned to the phylum Proteobacteria, 13% to the phylum Actinobacteria, 24% to the phylum Firmicutes, 21% to the phylum Nitrospirae and 4% to the phylum Verrucomicrobia.