Title of article
Antigenic Cross-Reactivity Anti-Birtoxin Antibody against Androctonus crassicauda Venom
Author/Authors
Van-Zoelen، SuhandanAdigüzel نويسنده Netherlands Organisation for Applied Scientific Research-Innovation for life-Hollanda, Turkey. Van-Zoelen, SuhandanAdigüzel , Ozkan، Ozcan نويسنده Experimental Research and Application Center, Drug and Medical Devices Agency, Ankara, Turkey. Ozkan, Ozcan , Inceoglu، Bora نويسنده Department of Entomology, University of California, Davis, USA. Inceoglu, Bora
Issue Information
فصلنامه با شماره پیاپی 0 سال 2015
Pages
8
From page
176
To page
183
Abstract
Background: Antivenom is still widely used in the treatment of envenomation as there are no vaccines or other effective agents available against animal venoms. Recently, neurotoxins named birtoxin family have been described from Parabuthus transvaalicus and Androctonus crassicauda. The aim of the present study was to test the antibirtoxin
antibodies for their ability to neutralize the lethal effects of A. crassicauda scorpion venom.
Methods: SDS-PAGE and Western blotting used the presence of components from A. crassicauda and P.transvaalicus scorpion venoms and to determine the degree of cross-reactivity. The Minimum Lethal Dose (MLD) of venom was assessed by subcutaneously (sc) injections in mice.
Results: The MLD of the A. crassicauda venom was 35 μg/ 20g mouse by sc injection route. Western blotting showed the presence of components from A. crassicauda and P. transvaalicus scorpion venoms strongly cross react with the A. crassicauda antivenom. However, Western blotting of the A. crassicauda scorpion venom using the Refik Saydam Public Health Agency (RSPHA) generated antibody showed that not all the venom components cross reacted with the anti-birtoxin antibody. The antibodies only cross reacted with components falling under the 19 kDa protein size of A. crassicauda venom.
Conclusion: The bioassays and Western blotting of A. crassicauda venom with the anti-birtoxin antibodies produced against a synthetic peptide showed that these antibodies cross reacted but did not neutralize the venom of A. crassicauda.
antibodies for their ability to neutralize the lethal effects of A. crassicauda scorpion venom.
Methods: SDS-PAGE and Western blotting used the presence of components from A. crassicauda and P.transvaalicus scorpion venoms and to determine the degree of cross-reactivity. The Minimum Lethal Dose (MLD) of venom was assessed by subcutaneously (sc) injections in mice.
Results: The MLD of the A. crassicauda venom was 35 μg/ 20g mouse by sc injection route. Western blotting showed the presence of components from A. crassicauda and P. transvaalicus scorpion venoms strongly cross react with the A. crassicauda antivenom. However, Western blotting of the A. crassicauda scorpion venom using the Refik Saydam Public Health Agency (RSPHA) generated antibody showed that not all the venom components cross reacted with the anti-birtoxin antibody. The antibodies only cross reacted with components falling under the 19 kDa protein size of A. crassicauda venom.
Conclusion: The bioassays and Western blotting of A. crassicauda venom with the anti-birtoxin antibodies produced against a synthetic peptide showed that these antibodies cross reacted but did not neutralize the venom of A. crassicauda.
Journal title
Journal of Arthropod-Borne Diseases
Serial Year
2015
Journal title
Journal of Arthropod-Borne Diseases
Record number
2386156
Link To Document