Assessment of Recombination in the S-segment Genome of Crimean-Congo Hemorrhagic Fever Virus in Iran
Chinikar، Sadegh نويسنده , , Shah-Hosseini، Nariman نويسنده Islamic Azad University, Lahijan Branch, Faculty of Science, Department of Microbiology, Lahijan, IR Iran , , Bouzari، Saeid نويسنده , , Shokrgozar، MohammadAli نويسنده National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran. Shokrgozar, MohammadAli , Mostafavi، Ehsan نويسنده Department of Epidemiology, Pasteur institute of Iran, Tehran, IR Iran , , Jalali، Tahmineh نويسنده Arboviruses and Viral Hemorrhagic Fevers Laboratory (National Reference Lab), Pasteur Institute of Iran, Tehran, Iran. Jalali, Tahmineh , Khakifirouz، Sahar نويسنده Arboviruses and Viral Hemorrhagic Fevers Laboratory (National Reference Lab), Pasteur Institute of Iran, Tehran, Iran. Khakifirouz, Sahar , Groschup، Martin H نويسنده Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany. Groschup, Martin H , Niedrig، Matthias نويسنده Robert Koch Institute, Berlin, Germany. Niedrig, Matthias
Background: Crimean-Congo Hemorrhagic Fever Virus (CCHFV) belongs to genus Nairovirus and family Bunyaviridae. The main aim of this study was to investigate the extent of recombination in S-segment genome of CCHFV in Iran.
Methods: Samples were isolated from Iranian patients and those available in GenBank, and analyzed by phylogenetic and bootscan methods.
Results: Through comparison of the phylogenetic trees based on full length sequences and partial fragments in the S-segment genome of CCHFV, genetic switch was evident, due to recombination event. Moreover, evidence of multiple recombination events was detected in query isolates when bootscan analysis was used by SimPlot software.
Conclusion: Switch of different genomic regions between different strains by recombination could contribute to CCHFV diversification and evolution. The occurrence of recombination in CCHFV has a critical impact on epidemiological investigations and vaccine design.