A long-lasting problem in the analysis of tissue samples is the time-consuming and repetitive process of histologic preparation and pathologic review of tissue sections. These two critical factors, innate nonuniformity in preparation and subjectivity of analysis, therefore limit the scientific and statistical thoroughness of tissue based studies. Employing tissue microarray (TMA) technique provides a potential solution to each of these problems. This review discusses the methods of creating tissue microarrays, the advantages and disadvantages of the technology, analysis methods, and recent TMA applications.