Title of article :
Quantitative Evaluation of BAFF, HMGB1, TLR 4 AND TLR 7 Expression in Patients with Relapsing Remitting Multiple Sclerosis
Author/Authors :
Hamid، Kabir Magaji نويسنده Department of Immunology, School of Public Health, Tehran University of Medical Sciences-International Campus, Tehran, Iran AND Department of Immunology, Faculty of Medical Laboratory Sciences, Usmanu Danfodiyo University, Sokoto, Nigeria Hamid, Kabir Magaji , Nejati، Ahmad نويسنده Department of Virology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran , , Shoja، Zabihollah نويسنده Department of Virology, Pasteur Institute of Iran, Tehran, Iran Shoja, Zabihollah , Mollaei-Kandelousd، Yaghoub نويسنده Immunology Department, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran Mollaei-Kandelousd, Yaghoub , Doosti، Rozita نويسنده , , Mirshafiey، Abbas نويسنده , , Tafakhori، Abbas نويسنده Assistant Professor of Neurology, Imam Khomeini Hospital, Iranian Center of Neurological Research , , Sahraian، Mohammad Ali نويسنده , , Marashi، Sayed Mahdi نويسنده Department of Forensic Medicine, School of Medicine, Tehran University of Medical ,
Abstract :
Multiple sclerosis is a chronic inflammatory disease of the central nervous system characterized by a complex immune response. Because of the complex nature of MS pathogenesis, a panel of biomarkers derived from different platforms will be required to reflect disease-related alterations. Monitoring and evaluation of molecules associated with the pathogenesis of the disease would provide useful information on disease progression and therapeutic assessment. In view of this, we evaluated the mRNA expression levels of B-cell activating factor (BAFF), high mobility group box 1 (HMGB-1), Toll like receptor (TLR) 4 and TLR7 in MS. These molecules are implicated in the pathogenesis of MS; however, they havereceived little attention. PBMCs were isolated from whole blood of 84 Relapsing Remitting Multiple Sclerosis patients and 70 healthy controls. Relative quantitative RT-PCR was applied to quantify the transcriptional levels of the immune markers. The mRNA expression levels of TLR7 were significantly elevated in RRMS patients than healthy controls. Whereas, TLR4 expression was found to be significantly lower in the patients than control group. We found no difference analyzing the mRNA levels of BAFF and HMGB1. Our data highlights the immune marker correlates in RRMS patients. However, further in-depth studies are warranted to check for their reliability of biomarkers in autoimmune diseases such as MS.
