Effect of Some Synthetic Parameters on Size and Polydispersity Index of Gelatin Nanoparticles Cross-Linked by CDI/NHS System

در اين پژوهش تغيير بعضي از پارامترها مانند نوع ژلاتين وفرمولاسيون عامل اتصال عرضي بر روي اندازه و توزيع نانوذرات ژلاتين اتصال عرضي شده با CDI/NHS مورد مطالعه قرار گرفت. براي سنتز نانوذرات روش دو مرحله اي حذف حلال مورد استفاده قرار گرفت و در نهايت مورفولوژي و متوسط اندازه به دست آمده مورد ارزيابي و مقايسه با نمونه هاي ديگر قرار گرفت. نتايج به دست آمده نشان داد كه مقادير زياد و نيز نبود NHS باعث افزايش اندازه نانوذرات وتوزيع پهن اندازه ذرات مي شود كه به دليل اتصال عرضي سريع و طولاني سيستم CDI/NHS مي باشد.

In our previous work, the effect of use of a water soluble CDI/NHS system as nontoxic cross-linking agent on fabrication of gelatin nanoparticles was investigated. In this research, the effect of variation in some synthetic parameters of gelatin nanoparticles cross-linked by CDI/NHS system such as type of gelatin and formulation of cross-linking agent on their size and distribution was examined. The conventional two step desolvation method was used for preparation of gelatin nanoparticles. The morphology, mean size and size distribution of the formed nanoparticles were evaluated and compared with each other. In addition, intrinsic viscosities of all the nanoparticles were measured and compared under different conditions. The results showed that the presence of more NHS and absence of NHS catalyst in CDI/NHS system lead to the large particle size and broad size distribution of nanoparticles that were attributed to the fast and slow cross-linking rate, respectively.