Increasing biocompatibility of scaffold made of Polyethersulfone (PES) through combining with polyaniline(PANI)

Today’s tissue engineering is an important and interesting method of treatment in bone lesions; hence, tissue engineering specialists and orthopedic surgeons are forced to serve as a safe alternative to current methods. The introduction of a scaffold that has the best efficiency is a major challenge to scientists and researchers in this field. The aim of this study is to investigate the polyaniline (PANI) influence on biocompatibility of Polyethersulfone (PES) nanofibrous scaffold. Fabricated scaffold was structurally characterized using SEM microscope and tensile assay. To investigate biocompatibility of nanofibers by MTT assay and DAPI staining, human mesenchymal stem cells were seeded on the surface of PES, PES-PANI and tissue culture polystyrene (TCPS) as control. SEM images demonstrated that the scaffolds were flat and the average diameters of the nanofibers were 286 ± 588. Moreover, the results of MTT assay and DAPI staining revealed that the highest proliferation rate of cells was observed in PES-PANI scaffold compared to the PES and TCPS as control. It can be concluded that PANI improves the three-dimensional structure of PES nanofibers and increases the biocompatibility of PES through support proliferation and penetration of MSCs in the PES nanofibers and can also be a good candidate for introduction and use in the bone tissue engineering application.