Detection of colon flora in peritoneal drain fluid after colorectal surgery: can RT-PCR play a role in diagnosing anastomotic leakage?
Van Leeuwen، Willem B. نويسنده Department of Surgery, Erasmus Medical Center Rotterdam, Rotterdam, The Netherlands AND Toplab, Leiden University of Applied Science, Niels Bohrweg 11-13, Leiden, The Netherlands. Van Leeuwen, Willem B. , Komen، N نويسنده Department of Surgery, Erasmus Medical Center Rotterdam, Rotterdam, The Netherlands AND Department of Surgery, ZNA Middelheim, Antwerp, Belgium. Komen, N , Morsink، MC نويسنده Toplab, Leiden University of Applied Science, Niels Bohrweg 11-13, Leiden, The Netherlands. Morsink, MC , Beiboer، S نويسنده Toplab, Leiden University of Applied Science, Niels Bohrweg 11-13, Leiden, The Netherlands. Beiboer, S , Miggelbrink، A نويسنده Toplab, Leiden University of Applied Science, Niels Bohrweg 11-13, Leiden, The Netherlands. Miggelbrink, A , Willemsen، P نويسنده Department of Surgery, ZNA Middelheim, Antwerp, Belgium. Willemsen, P , Van der Harst، E نويسنده Department of Surgery, Maasstad Ziekenhuis Rotterdam, Rotterdam, The Netherlands. Van der Harst, E , Lange، JF نويسنده Department of Surgery, Erasmus Medical Center Rotterdam, Rotterdam, The Netherlands. Lange, JF
Background and objectives: A semi-quantitative Real-Time PCR strategy was developed to identify indicator organisms for an indication on anastomotic leakage in peritoneal drainage fluid, Escherichia coli and Enterococcus faecalis.
Materials and Methods: The analytical performance of the amplification method was validated with 10 culture-positive and 7 culture negative peritoneal drain fluid samples, obtained from 9 different patients with a colorectal anastomosis.
Results: Real-Time PCR results were fully concordant with the microbiological culture results. However, among the culture negative samples, four false-positive RT-PCR results were found. All false-positives originated from a single patient with a surgical site infection. This may indicate an elevated sensitivity of the RT-PCR method.
Conclusion: The results showed that the semi-quantitative RT-PCR method has the clear potential to be useful as a powerful tool in early detection of anastomotic leakage.