Title of article
“In-house” production of DNA size marker from a vaccinal Bacillus anthracis strain
Author/Authors
Sekhavati، Mohammad Hadi نويسنده Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran , , Tadayon، Keyvan نويسنده Razi Vaccine and Serum Research Institute, Karaj, Iran Tadayon, Keyvan , Ghaderi، Rainak نويسنده Department of Veterinary Aerobic Bacteria, Razi Institute, Karaj, Iran. Ghaderi, Rainak , Banihashemi، Reza نويسنده Department of Veterinary Aerobic Bacteria, Razi Institute, Karaj, Iran. Banihashemi, Reza , Jabbari، AhmadReza نويسنده Department of Veterinary Aerobic Bacteria, Razi Institute, Karaj, Iran. Jabbari, AhmadReza , Shokri، Gholamreza نويسنده Department of Veterinary Aerobic Bacteria, Razi Institute, Karaj, Iran. Shokri, Gholamreza , Karimnasab، Nasim نويسنده Department of Microbiology, Faculty of Basic Sciences, Karaj Branch, Islamic Azad University, Karaj, IR Iran ,
Issue Information
فصلنامه با شماره پیاپی 0 سال 2015
Pages
5
From page
45
To page
49
Abstract
Background and Objectives: DNA molecular weight marker is widely used in molecular biology experiments incurring considerable costs on low-budget settings.
Materials and Methods: Here a PCR-supported procedure is described that uses 10 primer pairs targeting chromosomal DNA from the harmless vaccinal Bacillus anthracis Sterne 34F2 strain as template. A single PCR protocol is used to reproduce all the 10 fragments of a 100 bp DNA size marker.
Results and Conclusion: The unpurified amalgam of 10 PCR products can be directly loaded to agarose gels. This work was intended to develop a reasonably cost-effective DNA ladder that is useful for researchers in laboratories with limited funding.
Materials and Methods: Here a PCR-supported procedure is described that uses 10 primer pairs targeting chromosomal DNA from the harmless vaccinal Bacillus anthracis Sterne 34F2 strain as template. A single PCR protocol is used to reproduce all the 10 fragments of a 100 bp DNA size marker.
Results and Conclusion: The unpurified amalgam of 10 PCR products can be directly loaded to agarose gels. This work was intended to develop a reasonably cost-effective DNA ladder that is useful for researchers in laboratories with limited funding.
Journal title
IJM Iranian Journal of Microbiology
Serial Year
2015
Journal title
IJM Iranian Journal of Microbiology
Record number
2390249
Link To Document