Author/Authors :
Sekhavati، Mohammad Hadi نويسنده Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran , , Tadayon، Keyvan نويسنده Razi Vaccine and Serum Research Institute, Karaj, Iran Tadayon, Keyvan , Ghaderi، Rainak نويسنده Department of Veterinary Aerobic Bacteria, Razi Institute, Karaj, Iran. Ghaderi, Rainak , Banihashemi، Reza نويسنده Department of Veterinary Aerobic Bacteria, Razi Institute, Karaj, Iran. Banihashemi, Reza , Jabbari، AhmadReza نويسنده Department of Veterinary Aerobic Bacteria, Razi Institute, Karaj, Iran. Jabbari, AhmadReza , Shokri، Gholamreza نويسنده Department of Veterinary Aerobic Bacteria, Razi Institute, Karaj, Iran. Shokri, Gholamreza , Karimnasab، Nasim نويسنده Department of Microbiology, Faculty of Basic Sciences, Karaj Branch, Islamic Azad University, Karaj, IR Iran ,
Abstract :
Background and Objectives: DNA molecular weight marker is widely used in molecular biology experiments incurring considerable costs on low-budget settings.
Materials and Methods: Here a PCR-supported procedure is described that uses 10 primer pairs targeting chromosomal DNA from the harmless vaccinal Bacillus anthracis Sterne 34F2 strain as template. A single PCR protocol is used to reproduce all the 10 fragments of a 100 bp DNA size marker.
Results and Conclusion: The unpurified amalgam of 10 PCR products can be directly loaded to agarose gels. This work was intended to develop a reasonably cost-effective DNA ladder that is useful for researchers in laboratories with limited funding.
