Prevalence assessment of magA gene and antimicrobial susceptibility of Klebsiella pneumoniae isolated from clinical specimens in Shahrekord, Iran.
Amraie، Hadis نويسنده Department of Microbiology, Lorestan University of Medical Sciences, Khorramabad, Iran. Amraie, Hadis , Shakib، Pegah نويسنده Cellular and Molecular Research Center, Microbiology Department, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, IR Iran Shakib, Pegah , Rouhi، Samaneh نويسنده Cellular and Molecular Research Center, Microbiology Department, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, IR Iran Rouhi, Samaneh , Bakhshandeh، Neda نويسنده Applied Agriculture Branch, Semnan Applied Sciences and Technology University, Semnan, Iran. Bakhshandeh, Neda , Zamanzad، Behnam نويسنده Medical Plant Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran Zamanzad, Behnam
Background and Objectives: Klebsiella pneumoniae (K. pneumoniae) is an opportunistic microorganism. This study aimed to investigate the presence of magA gene and antimicrobial susceptibility in K. pneumoniae.
Materials and Methods: 195 clinical specimens were collected from hospitals of Shahrekord, Iran. Bacterial culture, biochemical diagnostic standard test, determination of antibiotic sensitivity, phenotypic testing hypermucoviscosity (HV) and polymerase chain reaction (PCR) was performed for isolation and characterization of K. pneumoniae.
Results: 173 samples were positive for K. pneumoniae. The highest and lowest rates of resistance were related to amoxicil-lin 79.19% and ciprofloxacin 15.60%, respectively. Also 4 samples were positive for magA gene.
Conclusion: Based on our results, K. pneumoniae strains were resistant to different antibiotics. Knowing how to identify strains of K. pneumoniae, spreading of its virulence and also antimicrobial resistance genes can be useful in treatment of infection caused by this bacterium.