Author/Authors :
Mohammadkhani، Nayer نويسنده Department of Biology, Faculty of Science, Urmia University, Urmia, Iran , , Heidari، Reza نويسنده Department of Biology, Faculty of Science, Urmia University, Urmia, Iran , , Abbaspour، Nasser نويسنده Department of Biology, Faculty of Science, Urmia University, Urmia, Iran , , Rahmani، Fatemeh نويسنده Department of Biology, Faculty of Science, Urmia University, Urmia, Iran ,
Abstract :
اطلاعات مولكولي انباشتگي K+ در انگور مورد نياز است، تحت شوري ناقلين پتاسيمي به وسيله Na+ در انگور مهار مي شوند. هدف اين مطالعه بررسي اثرات شوري بر بيان ژن هاي ناقلين K+ در شرايط شوري در انگور بود. بر اساس مطالعه غربالگري ما روي 18 ژنوتيپ انگور"H6" و "قره شاني" (متحمل) و "شيرازي" و "قزل اوزوم" (حساس) انتخاب شدند. گياهان با mM NaCl 50 به عنوان يك غلظت بحراني كه براي گياهان انگور كشنده نيست، تيمار شدند. به طور جالبي بيان ژن هاي VvKUP1، VvKUP2 و VvK1.1 در برگ هاي ژنوتيپ هاي حساس بيشتر از متحمل بود. همچنين بيان ژن هايVvKUP1 و VvKUP2 در برگ ژنويپ هاي حساس مشابه بود. همبستگي مثبت معني داري(P < 0.05) بين بيان ناقلين K+و انباشتگي Na+ در برگ هاي ژنوتيپ هاي حساس وجود داشت. يافته هاي ما يك ارتباط قوي بين انباشتگي نسخه هاي خاص و درجه تحمل به تنش نشان داد.
Abstract :
Molecular information of K+ accumulation in grapes is strongly required. Under salinity condition potassium transporters are inhibited by Na+. The aim of this study was to investigate the effects of salinity on the expression of K+ transporter genes in grape. Based on the previous screening study on 18 grape genotypes, ‘H6’ and ‘Gharashani’ (tolerant) and ‘Shirazi’ and ‘GhezelUzum’ (sensitive) were selected. Plants were treated with 50 mM NaCl as a critical concentration that was not lethal for grapevine plants. Interestingly, the expression of VvKUP1, VvKUP2 and VvK1.1 genes highly increased in leaves of sensitive genotypes compared to tolerant ones. Also the expression of VvKUP1 and VvKUP2 genes were similar in the leaves of sensitive genotypes. There was a significant positive correlation (P < 0.05) between the expression of K+ transporters –VvKUP1 and VvKUP2- and the accumulation of Na+ in the leaves of sensitive genotypes. Roots of all genotypes showed increase in expression of VvK1.1 under salinity. The findings highlighted a strong relationship between the accumulation of specific transcripts and the degree of stress tolerance.
