Author/Authors :
Falahati ، M نويسنده Department of Medical Mycology and Parasitology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran , , Sepahvand ، A نويسنده Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran , , Mahmoudvand ، H نويسنده Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran , , Baharvand ، P نويسنده Department of Biostatistics, School of Health and Nutrition, Lorestan University of Medical Sciences, Khorramabad, Iran , , Jabbarnia ، S نويسنده Razi Vaccine and Serum Research Institute, Tehran, Iran , , Ghojoghi ، A نويسنده Department of Medical Mycology and Parasitology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran , , Yarahmadi ، M نويسنده Department of Medical Mycology and Parasitology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran ,
Abstract :
Background and Purpose:
Despite the availability of various treatments for fungal diseases, there are some limitations
in the management of these conditions due to multiple treatment
-related side
-effects. The present study was designed to
investigate the an
tifungal properties of different extracts from
Pistacia atlantica
Desf
.
Materials and Methods:
Different parts of
P. atlantica
(i.e., dried fruit, fresh fruit and dried leaf) were separately
extracted via percolation method with 80% methanol and water.
Gas
chromatography/mass spectrometry (GC/MS)
analysis was performed to determine the main constituents of leaf and fruit extracts from
P. atlantica
.
In vitro
anti
-
Candida
activities of the extracts against
Candida albicans
,
Candida glabrata
and
Saccharomyces
cerevisiae
were
studied. For this purpose, the minimum inhibitory concentrations (MICs) and minimum fungicidal concentrations
(MFCs) were determined, using broth microdilution method, according to the modified M27-
A3 protocol on yeasts,
proposed
by the Cli
nical and Laboratory Standards Institute (CLSI)
.
Results:
Based on GC/MS analysis, the main constituents of
P. atlantica
fruit extracts were B
-
myrcene (41.4%), ?
-
pinene (32.48%) and limonene (4.66%), respectively, whereas the major constituents of
P. atla
ntica
leaf extracts were
trans
-
caryophyllene (15.18%), ?
-amorphene (8.1%) and neo-
allo
-
ocimene (6.21%), respectively. As the findings
indicated, all the constituents exhibited both fungistatic and fungicidal activities, with MICs ranging from 6.66 to 26.66
mg/mL and MFCs ranging from 13.3 to 37.3 mg/mL, respectively. Among the evaluated extracts, the methanolic fresh
fruit extract of
P. atlantica
was significantly more effective than other extracts (P < 0.05)
.
Conclusion:
Based on the findings of the present study, novel antifungal agents need to be developed, and use of
P.
atlantica
should be promoted in the traditional treatment of
Candida
infections
.
