Author/Authors :
Saghi، Hossein نويسنده AJA University of Medical Sciences, Tehran, IR Iran , , Amiri Dastjerdi، Ferdoes نويسنده AJA University of Medical Sciences, Tehran, IR Iran , , Zahedi، Bahador نويسنده Department of Microbiology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, IR Iran , , Mohsen Pour، Mohammad نويسنده Department of Microbiology, Baqiyatallah University Medical of Sciences, Tehran, IR Iran , , Khorrami، Mahmoud نويسنده AJA University of Medical Sciences, Tehran, IR Iran , , Efati، Majid نويسنده AJA University of Medical Sciences, Tehran, IR Iran , , Esmaeili، Davoud نويسنده Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran ,
Abstract :
Acinetobacter baumannii is one of the Gram-negative bacteria in nosocomial infections that is resistant to treatment and control. The isolation of these bacteria is increasing significantly throughout the world. With increasing populations and a reduction in the use of synthetic drugs, many of these medicinal herbs are replacing other treatments. The present study aims to investigate the inhibitory effects of Satureja khuzestaniea essence on the expression of the antibiotic resistance genes bla-OXA-23 and Housekeeping DNA gyrase-A in multidrug-resistant strains of Acinetobacter baumannii, using the RT-PCR technique. Satureja khuzestaniea essential oil was collected from the Barij essence research farm in May 2010. Five multidrug-resistant strains of Acinetobacter baumannii that contain the bla-OXA-23 and housekeeping DNA gyrase-A genes were selected. The disk diffusion method was used to evaluate the antimicrobial effect of essential oil, and major components were used. The minimum inhibitory concentration (MIC) values of the Satureja khuzestaniea essential oil were determined via a broth macro dilution assay, as recommended by the CLSI. Finally, an analysis of the bla-OXA-23 and Housekeeping DNA gyrase-A was performed using the RT-PCR technique. The major components of Satureja khuzestaniea essence are carvacrol (90.88%), ρ-cymene (3.11%), γ terpinene (1.24%), and linalool (0.91%). The RT-PCR technique demonstrates that Satureja khuzestaniea with an MIC of 0.2 μL/mL has the effect of reducing the expression of the antibiotic resistance gene bla-OXA-2. The essence has no inhibitory effect, however, on the Housekeeping DNA gyrase-A gene. This study indicates that Satureja khuzestaniea essence has inhibitory effects on the gene expression of antibiotic resistance in bla-OXA-23 that has a high MIC. Given that this essence has a good inhibitory effect on gene expression in MDR Acinetobacter baumannii and bla-OXA-23, the results indicate that it could be used as a natural way to prevent the growth of Acinetobacter baumannii.
