Title of article :
Comparison of four different culture media for growth of Mycobacterium avium subsp. avium isolated from naturally infected lofts of domestic pigeons.
Author/Authors :
Mayahi، Mansour نويسنده Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran , , Mosavari، Nader نويسنده Department of Microbiology, Razi Vaccine & Serum Research Institute, Tehran University of Medical Sciences, Tehran, Iran Mosavari, Nader , Esmaeilzadeh، Saleh نويسنده University of Ahvaz , , parvandar asadollahi، Kaveh نويسنده Shahid Chamran University of Ahvaz ,
Issue Information :
فصلنامه با شماره پیاپی 0 سال 2013
Pages :
4
From page :
379
To page :
382
Abstract :

Background and Objectives: Diagnosis of avian tuberculosis by conventional culture method is still considered as the “gold standard” technique. The main objective of this study was to compare growth of Mycobacterium avium subsp. avium on four specific Mycobacterial cultures such as glycerinated Lowenstein-Jensen medium, pyruvate-enriched Lowenstein- Jensen medium, mycobactin J-supplemented Herrold-egg yolk medium and plain Herrold-egg yolk medium.
Materials and Methods: Eighty out of more than 600 pigeons were selected based on their clinical signs and poor health conditions. The birds were numbered and their clinical signs were registered in the working sheets, and under standard condi- tion, euthanized, subjecting to necropsy examinations, followed by bacterial culture on four specific media for Mycobacterium avium subsp. avium, including glycerinated Lowenstein-Jensen (LJG) medium, pyruvate-enriched Lowenstein-Jensen medium (LJP), mycobactin J-supplemented Herrold-egg yolk medium and plain Herrold-egg yolk medium.
Results: Fifty one Mycobacterium avium subsp. avium were isolated from pigeons. Mycobactin J-supplemented Herrold-egg yolk media yielded greater number of colonies in shorter incubation time in compare with  other media.
Conclusion:  It was concluded that most of the isolates need mycobactin as a growth factor.

Journal title :
IJM Iranian Journal of Microbiology
Serial Year :
2013
Journal title :
IJM Iranian Journal of Microbiology
Record number :
2392412
Link To Document :
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