Author/Authors :
Jalali، Hanieh نويسنده Department of Developmetal Biology, Faculty of Biological Science, Kharazmi University, Tehran, Iran , , Parivar، Kazem نويسنده , , Soleimani، Masoud نويسنده , , Nabiuni، Mohammad نويسنده Cell and Developmental Biology, Biology Department, Tarbiat Moallem University, Tehran, Iran , , Aghaee-Bakhtiari، Hamid نويسنده Department of Molecular Biology and Genetic Engineering, Stem Cells Technology Research Center, Tehran, IR Iran ,
Abstract :
Background
Introduction of therapeutic genes into the injured site of nervous system can be achieved using transplantation of cellular vehicles containing desired gene. To transfer exogenous genes into the cellular vehicles, lentiviral vectors are one of interested vectors because of advantages such high transduction efficiency of dividing and non-dividing cells. Unrestricted somatic stem cells are subclasses of umbilical cord blood derived stem cells which are appreciate candidates to use as cellular vehicles for ex vivo gene therapy of nervous system.
Objectives
In current study we investigated the effect of lentiviral vector transduction on the neuronal related features of unrestricted somatic stem cells to indicate the probable and unwanted changes related to transduction procedure.
Materials and Methods
In this experimental study, lentiviral vector containing green fluorescent protein (GFP) were transduced into unrestricted somatic stem cells and its effect was investigated with using MTT assay, qPCR and immunohistochemistry techniques. For statistical comparison of real time PCR results, REST software (2009, Qiagen) was used.
Results
Obtained results showed lentiviral vector transduction did not have cytotoxic effects on unrestricted somatic stem cells and did not change neuronal differentiation capacity of them as well the expression of some neuronal related genes and preserved them in multilineage situation.
Conclusions
In conclusion, we suggested that lentiviral vectors could be proper vectors to transfer therapeutic gene into unrestricted somatic stem cells to provide a cellular vehicle for ex vivo gene therapy of nervous system disorders.