Author/Authors :
Arbabi، Leila نويسنده Antimicrobial Resistance Research Center, Rasoul-e-Akram Hospital, Iran University of Medical Sciences, Tehran, IR Iran Arbabi, Leila , Boustanshenas، Mina نويسنده Science and Research Branch, Islamic Azad University, Tehran, Iran , , Rahbar، Mohammad نويسنده Antimicrobial Resistance Research Center, Rasoul-e-Akram Hospital, Iran University of Medical Sciences, Tehran, IR Iran Rahbar, Mohammad , Owlia، Parviz نويسنده , , Adabi، Maryam نويسنده Antimicrobial Resistance Research Center, Tehran University of Medical Sciences, Tehran, IR Iran. Adabi, Maryam , Rasouli Koohi، Samira نويسنده Antimicrobial Resistance Research Center, Rasoul-e-Akram Hospital, Iran University of Medical Sciences, Tehran, IR Iran Rasouli Koohi, Samira , Afshar، Mastaneh نويسنده Antimicrobial Resistance Research Center, Rasoul-e-Akram Hospital, Iran University of Medical Sciences, Tehran, IR Iran Afshar, Mastaneh , Fathizadeh، Sara نويسنده Antimicrobial Resistance Research Center, Rasoul-e-Akram Hospital, Iran University of Medical Sciences, Tehran, IR Iran Fathizadeh, Sara , Majidpour، Ali نويسنده Antimicrobial Resistance Research Center, Rasoul-e-Akram Hospital, Iran University of Medical Sciences, Tehran, IR Iran Majidpour, Ali , Talebi-Taher، Mahshid نويسنده Antimicrobial Resistance Research Center, Rasoul-e-Akram Hospital, Iran University of Medical Sciences, Tehran, IR Iran Talebi-Taher, Mahshid
Abstract :
Background: Enterococcus spp. are part of the normal flora of humans and animals. The nosocomial pathogenicity of Enterococcus
spp. has emerged in recent years and has caused great concern due to developing of resistance to many antimicrobial agents.
Objectives: The current study aimed to determine the resistance pattern and the type of virulence genes in Enterococcus spp. isolated
from Milad hospital of Tehran, Iran.
Materials and Methods: The current observational study was conducted from Apr 2014 to Feb 2015 on a total of 149 Enterococcus
species isolated from Milad hospital in Tehran, Iran. The antibiotic susceptibility pattern of the bacteria was determined by the disc
diffusion method for eight antibiotics. Minimum inhibitory concentration (MIC) of vancomycin was also done using agar-dilution
assay by clinical and laboratory standards institute (CLSI) recommendations. The sodA, esp, cyl, ace and gelE genes were detected by
polymerase chain reaction (PCR) assay.
Results: About 37.5%, 73%, 86.6%, 35.8%, 69%, 60.8%, 45% and 79% of the isolates were resistant to vancomycin, tetracycline, gentamicin,
chloramphenicol, ciprofloxacin, penicillin, ampicillin and erythromycin, respectively. MIC on 38% of the isolates was 256
g/mL. Although, the prevalence of vancomycin-resistant Enterococcus (VRE) strains belonged to two species, E. faecium showed high
resistance to a broad range of antibiotics. In total, 94 isolates were positive for esp, and 59, 48 and 3 isolates were positive for ace,
cylA and gelE, Respectively.
Conclusions: The results of the current study designate the important role of medical samples as reservoirs of resistance inducing
elements. Early detection of VRE with their virulence trait will help to prevent the spread of vancomycin resistant Enterococcus
species. Supervision for antibiotic usage in hospitals, especially for last option antibiotics, can prevent the spread of resistant isolates
and losing all treatment options in the future.
