Author/Authors :
Minayi، Neda نويسنده Department of Hematology, Faculty of Paramedical Sciences, Tehran University of Medical Sciences, Tehran, Iran. , , Alizadeh، Shaban Ali نويسنده Department of Surgery, School of Medicine, Arak University of Medical Sciences, Arak, Iran , , Dargahi، Hosein نويسنده Health care services Department, Medical School, Tehran University of Medical sciences, Tehran, Iran. Dargahi, Hosein , Soleimani، Masoud نويسنده , , Khatib، Zahra Kashani نويسنده BSc of CLS , , Tayebi، Behnoosh نويسنده Department of Hematology, Qaem Hospital, Mashhad University of Medical Sciences , , Mohammadian، Mozhde نويسنده Hematology Department, Tabriz University of Medical sciences, Tabriz, Iran. Mohammadian, Mozhde , Alijani، Sadegh نويسنده Department of Animal Sciences, Faculty of Agriculture, University of Tabriz, Tabriz, Iran , , Karami، Farshid نويسنده Department of Hematology, Allied Medical School, Tehran University of Medical Sciences ,
Abstract :
Background: Bone marrow derived mesenchymal stem cells (MSCs) are a population of multipotent progenitors which have the capacity of proliferation and differentiation into mesenchymal lineage cells. Hypoxia could promote the proliferation of MSCs. Micro-RNAs are endogenous RNAs that can play an important role in some processes such as proliferation and differentiation. MiR-210 could help for better proliferation of MSCs since this miRNA could activate HIF pathway. In current study we investigated if MSCs can preserve their differentiation and proliferation ability under normoxic conditions by upregulation of miR-210.
Materials and Methods: MSCs isolated from C57 BL/6 mice by flushing itʹs femurs into the cell culture media. After 72 hours, MSCs which are plastic adherent cells were attached to the flask and non-adherent cells were removed. Subsequently, MSCs induced to differentiate into osteocytes and adipocytes with specific differentiation media in order to confirm their identity and multipotency. Then miR-210 was inserted in Lentiviruse vectors and affected MSCs. In each passage, the number and viability of cells were evaluated.
Results: Comparison between miR-210 infected MSCs with control cells showed that miR-210 has ability to increase proliferation of MSCs significantly.
Conclusion: We showed that miR-210 has ability to induce proliferation of MSCs without any negative effect on their differentiation abilities. Further studies are needed for evaluation of probable effects of miR-210 mechanisms on MSCs proliferation.
