Assessment of in vitro chromosomal sensitivity to low doses of gamma irradiation in patients with acute lymphoblastic leukemia
Ramyar، Asghar نويسنده , , Aghamohammadi، Asghar نويسنده , , Mozdarani، Hossein نويسنده , , MAHMOODI، MAJID نويسنده , , Azimi، Cyrus نويسنده Genetics Group, Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran. , , Safari Kahreh، Zahra نويسنده , , Nedaei، Hassan-Ali نويسنده Radiotherapy Oncology Department, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran Nedaei, Hassan-Ali , Farzanfar، Fareedeh نويسنده Genetics Group, Cancer Research Center, Cancer Institute,Tehran University of Medical Sciences, Tehran, Iran Farzanfar, Fareedeh , Rezaei، Nima نويسنده , , Asadi-Shekaari، Majid نويسنده Electron Microscopy Department, Kerman Neuroscience Research Center (KNRC), Kerman, Iran , , Esfahani، Mahbod نويسنده Radiotherapy Oncology Department, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran Esfahani, Mahbod , Hosseini، Seyed Kazem نويسنده Cancer Research Center, Tehran University of Medical Sciences, Tehran, Iran Hosseini, Seyed Kazem , Yazdani، Mahdi نويسنده Radiotherapy Oncology Department, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran Yazdani, Mahdi , Abolhassani، Hassan نويسنده , , Khalesi، Fatemeh نويسنده Radiotherapy Oncology Department, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran Khalesi, Fatemeh , Mohagheghi، Mohamad Ali نويسنده Cancer Research Center, Tehran University of Medical Sciences, Tehran, Iran Mohagheghi, Mohamad Ali
Background: Acute lymphoblastic leukemia (ALL) constitutes a heterogeneous group of diseases characterized by abnormal proliferation and accumulation of immature lymphoblasts arrested at various differentiation stages. Increasing evidence suggests that chromosomal defects are present in these patients. The aim of this study is to investigate the chromosomal radiosensitivity in a group of ALL patients.
Methods: To analyze chromosomal radiosensitivity of ALL patients, lymphocytes of 20 patients were cultured followed by exposure to γ-ray irradiation to detect the chromosomal aberrations as an indicator of radiosensitivity. Cells were scored for the number of aberrations (chromatid breaks, chromatid gaps, chromosome breaks, chromosome gaps and chromatid exchanges). Results were compared with healthy individuals, and ataxia telangiectasia (AT) patients as positive control.
Results: On average number of aberrations in ALL patients was significantly higher than that in healthy controls. Â We found 65% of ALL patients appeared to be susceptible to in vitro irradiation. Chromosomal radiosensitivity of 35% patients was not different from healthy donors. Ataxia telangiectasia patients showed the highest degree of lymphocyte radiosensitivity. The results also indicate that there is a good correlation between the two assays, G2 and G0, using the same blood sample for both assays.
Conclusion: According to the result, we concluded that most of the ALL patients are sensitive to ionizing radiation and therefore should be protected from unnecessary diagnostic and therapeutic procedures using ionizing radiation.