Author/Authors :
Biyikli، Oguz Oben نويسنده Clinic of Chest Diseases, Kusadasi Universal Hospital, Aydin, Turkey , , Baysak، Aysegul نويسنده Chest Diseases Department, School of Medicine, Izmir University, Izmir, Turkey , , Ece، Gulfem نويسنده Department of Medical Microbiology, Izmir University, School of Medicine, Izmir, Turkey , , Oz، Adnan Tolga نويسنده Chest Diseases Department, School of Medicine, Izmir University, Izmir, Turkey , , Ozhan، Mustafa Hikmet نويسنده Chest Diseases Department, School of Medicine, Ege University, Izmir, Turkey , , Berdeli، Afig نويسنده Department of Pediatrics, Molecular Medicine Laboratory, Ege University, Izmir ,
Abstract :
One-third of the world’s population is infected with Mycobacterium tuberculosis. Investigation of Toll-like receptors (TLRs) has revealed new information regarding the immunopathogenesis of this disease. Toll-like receptors can recognize various ligands with a lipoprotein structure in the bacilli. Toll-like receptor 2 and TLR-4 have been identified in association with tuberculosis infection. The aim of our study was to investigate the relationship between TLR polymorphism and infection progress. Twenty-nine patients with a radiologically, microbiologically, and clinically proven active tuberculosis diagnosis were included in this 25-month study. Toll-like receptor 2 and TLR-4 polymorphisms and allele distributions were compared between these 29 patients and 100 healthy control subjects. Peripheral blood samples were taken from all patients. Genotyping of TLR-2, TLR-4, and macrophage migration inhibitory factor was performed. The extraction step was completed with a Qiagen mini blood purification system kit (Qiagen, Ontario, Canada) using a peripheral blood sample. The genotyping was performed using polymerase chain reaction-restriction fragment length polymorphism. In total, 19 of the 29 patients with tuberculosis infection had a TLR-2 polymorphism, and 20 of the 100 healthy subjects had a TLR-2 polymorphism (P < 0.001). The TLR-4 polymorphism and interferon-γ allele distributions were not statistically correlated. Toll-like receptor 2 polymorphism is a risk factor for tuberculosis infection. The limiting factor in this study was the lack of investigation of the interferon-γ and tumor necrosis factor-α levels, which are important in the development of infection. Detection of lower levels of these cytokines in bronchoalveolar lavage specimens, especially among patients with TLR-2 defects, will provide new data that may support the results of this study.
