Author/Authors :
Mortezagholi، Sahar نويسنده Department of Immunology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. Mortezagholi, Sahar , Babaloo، Zohreh نويسنده Drug Applied Research Center, Tabriz, Iran, Tabriz University of Medical Sciences, Tabriz, Iran. , , Rahimzadeh، Parisa نويسنده Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Rahimzadeh, Parisa , Ghaedi، Mojgan نويسنده Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Ghaedi, Mojgan , Namdari، Hayedeh نويسنده Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Namdari, Hayedeh , Assar، Shirin نويسنده Rheumatology Research Center, Tehran University of Medical Sciences, Tehran, Iran Assar, Shirin , Azimi Mohamadabadi، Maryam نويسنده Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Azimi Mohamadabadi, Maryam , Salehi، Eisa نويسنده Department of Immunology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran Salehi, Eisa
Abstract :
Systemic lupus erythematosus (SLE) is a chronic autoimmune disease which results in damage to various organs. Some animal studies have revealed that activation of Toll-like receptors (TLRs) is important in the pathogenesis of SLE. In the present study, the percentage of different immune cell subsets in 35 SLE patients and 38 control subjects was analyzed by flow cytometry. We also assessed the expression of TLR9 in the population of peripheral blood mononuclear cells (PBMCs) including T lymphocytes (CD4+ and CD8+), B lymphocytes (CD19+), NK cells (CD56+) and monocytes (CD14+) in SLE patients and healthy controls. The results showed that the percentage of CD8+ T lymphocytes and CD14+ monocytes were significantly higher (p˂0.001) in the SLE patients than the healthy control subjects. Moreover, the percentage of CD56+ NK cells were significantly lower in the SLE patients than the healthy control subjects (p=0.001). The findings indicated that the expression of TLR9 was significantly higher in CD4+ and CD8+ T lymphocytes and CD19+ B lymphocytes of SLE patients than in control subjects (all p˂0.05). The difference in TLR9 expression are involved in pathogenesis of the SLE, hence it can be used as an indicator for SLE diagnosis.
