Author/Authors :
Farrokhi، Mehrdad نويسنده Department of Environmental Health Engineering, Faculty of Health, Guilan University of Medical Sciences, Guilan, Iran Farrokhi, Mehrdad , Dabirzadeh، Mehrnoosh نويسنده Department of Neurology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Dabirzadeh, Mehrnoosh , Dastravan، Nastaran نويسنده Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran Dastravan, Nastaran , Etemadifar، Masoud نويسنده , , Ghadimi، Keyvan نويسنده Department of Neurology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Ghadimi, Keyvan , Saadatpour، Zahra نويسنده Department of Radiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Saadatpour, Zahra , Rezaei، Ali نويسنده Department of Biology, Faculty of Sciences, Arak University, Arak 38156-8-8349, Iran, Fax: +98 861 4173406, ,
Abstract :
Multiple sclerosis (MS) is a complex, demyelinating disease of the central nervous system (CNS) with variable phenotypic presentations, while Guillain-Barre Syndrome (GBS) is the prototypic acute inflammatory disorder that affects the peripheral nervous system. Myasthenia gravis (MG) is a T cell dependent and antibody mediated autoimmune disease. Although it has been shown that complement plays a critical role in the pathogenesis of MS, GBS, and MG, the role of mannose-binding lectin (MBL) as a biomarker of immunopathogensis of these diseases and also its association with the severity of them have been poorly investigated. Therefore, in this study we aimed to measure plasma levels of MBL in patients with MS, GBS, and MG. In a case-control study, plasma was obtained from healthy controls (n=100) and also patients with MS (n=120), GBS (n=30), and MG (n=30). Plasma level measurement of MBL was performed using enzyme-linked immunosorbent assay (ELISA). The mean serum level of MBL was significantly different between groups of patients and healthy controls (p<0.001). We also found a positive correlation between plasma levels of MBL and severity scores of MS, MG, and GBS patients including: expanded disability status scale (EDSS) (r=+0.60 and p=<0.001), quantitative myasthenia gravis score (QMGS) (r=+0.56 and p=0.01), and GBS disability scale (GDS) (r=+0.37 and p=0.04). Taken together, our findings suggest that complement activation mediated by MBL contributes to the pathogenesis and also severity of MS, MG, and GBS. However, because the lectin pathway can be involved in several phases of the immune response, further evidence will be required to elucidate the underlying mechanism.
