Sol-gel Approach for Fabrication of Solid-Phase Microextraction Hollow Fiber: An Efficient Adsorbent for Enrichment of Trace Levels of Antidepressant Drug, Fluoxetine

اين تحقيق عبارتست از توسعه دستورالعملي براي تعيين مقادير كم داروي فلوكسيتين در نمونه هاي آبي. روش بر پايه استفاده از جاذب سيليكاي بر پايه سل-ژل مي باشد كه براي استخراج اين دارو از نمونه هاي آبي به كار رفته است. روش استخراج هم، استفاده از ميكرواستخراج فاز جامد فيبر توخالي مي باشد. تحت شرايط بهينه فاكتور پيش تغليظ و حد تشخيص بدست آمده عبارتند از 3227 و 53/0 نانوگرم بر ميلي ليتر. رنج خطي عيارت بود از 0/1 تا 0/10000 نانوگرم بر ميلي ليتر و تكرار پذيري نيز بر مبناي درصد انحراف استاندارد نسبي 8/4 درصد بدست آمد.

This research is on the improvement of the procedure to determination of trace levels of antidepressant drug; fluoxetine in wastewaters. In this research a silica based sol-gel was applied for the extraction of fluoxetine from water samples. This two-phase technique is consisting of aqueous samples of fluoxetine (donor phase) and silica based nanocomposite prepared by sol-gel technique (acceptor phase). Accepter phase was held in the pores and lumen of polypropylene hollow fiber segment. Microextraction experiments were carried out in two steps; extraction from analyte samples by sorbent which is held into the hollow fiber segment and desorption of drug from hallow fiber by using of methanol. Desorbed analyte in order to measurement were offered to HPLC and UV-V is spectrophotometer for further analysis. This method is simple, fast and adopted by a majority of the instrumental methods. Extraction parameters such as sol-gel aging time, pH of donor phase, volume of donor phase, extraction time, stirring rate and effect of surfactant were investigated and optimized. The measurements were done under the optimal conditions. This technique has many advantages, such as the short extraction time, low consumption of organic solvents, elimination of carry-over effect, low limit of detection and high pre-concentration factor. The pre-concentration factor and limit of detection have been gained 3227 and 0.53 ng mL-1, respectively. The linear range and relative standard deviation are 1.0-10000.0 ng mL-1 and 4.8% (n=3), respectively.