Detection and Molecular Characterization of Cryptosporidium species in Recreational Waters of Chaharmahal va Bakhtiyari Province of Iran using nested-PCR-RFLP
Manouchehri Naeini، K نويسنده Department of Parasitology, Mycology and Entomology, School of Medicines, Shahr-e-kord University of Medical Science, Shahr-e-kord, Iran Manouchehri Naeini, K , Asadi، M نويسنده Department of Parasitology, Mycology and Entomology, School of Medicines, Shahr-e-kord University of Medical Science, Shahr-e-kord, Iran Asadi, M , Hashemzade Chaleshtori، M نويسنده Cellular and Molecular Research Center, Shahr-e-kord University of Medical Sciences, Shahrekord,Iran Hashemzade Chaleshtori, M
Background: The aim of this study was to detect and characterize Cryptosporidium spp. in water samples collected from recreational ponds of Chaharmahal va Bakhtiyari Province of Iran .
Methods: Thirty water samples were collected from November 2009 to May 2010. Each sample contained 10 liters of water. We used the SSU rRNA-based PCR-RFLP technique.
Results: Out of thirty samples examined, 6 (20%) were positive for different Cryptosporidium spp. Restriction pattern analysis showed that C. parvum has been the most prevalent genotype, followed by C. hominis and C. canis , respectively. In this area, the higher prevalence of C. parvum compared with other genotypes is consistent with the distribution of cattle.
Conclusion: Farm animals, particularly cattle arethe main source of cryptosporidial contamination for recreational waters in this area.