Title of article :
Protective role of Lactobacillus plantarum A7 against irinotecan-induced genotoxicity
Author/Authors :
Sepahi، Soheila نويسنده Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences,Department of Pharmacology,Isfahan University of Medical Sciences,Isfahan,Iran , , Jafarian-Dehkordi، Abbas نويسنده Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences,Department of Pharmacology,Isfahan University of Medical Sciences,Isfahan,Iran , , Mirlohi، Maryam نويسنده Food Security Research Centre, School of Nutrition and Food Sciences,Isfahan University of Medical Sciences,Isfahan,Iran , , shirani، kobra نويسنده Faculty of Pharmacy,Department of Pharmacodynamy and Toxicology,Mashhad University of Medical Sciences,Mashhad,Iran , , Etebari، Mahmoud نويسنده Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences,Department of Pharmacology,Isfahan University of Medical Sciences,Isfahan,Iran ,
Issue Information :
دوماهنامه با شماره پیاپی سال 2016
Pages :
7
From page :
329
To page :
335
Abstract :
Objective: Irinotecan is a botanical derivative and an anticancer drug with cytotoxic and genotoxic effects. The present study evaluated the effect of Lactobacillus plantarum A7 on the genotoxic activity of irinotecan in a hepatocellular carcinoma cell line (HepG2) by comet assay. Materials and Methods: HepG2 were incubated with irinotecan (100 µM), heatkilled cells (0.025 µg/ml) + irinotecan (100 µM), and cellfree supernatants (0.5 and 1 µg/ml) of L. plantarum A7 + irinotecan (100 µM). Phosphate buffered saline (PBS) was used as negative control. Results: Irinotecan was shown to induce DNA damage in HepG2 cells. The results showed that heatkilled cells (0.025 µg/ml) and cellfree supernatants (0.5 and 1 µg/ml) of L. plantarum significantly reduce irinotecan induced DNA damage. Conclusion: Our results indicate that L. plantarum A7 can decrease the genotoxic effects of irinotecan in HepG2 cells, in vitro. This finding may be supportive for the optimization of therapeutic efficacy in irinotecan treatment.
Keywords :
irinotecan , Lactobacillus plantarum A7 , anti-genotoxicity , HepG2 , Comet assay
Journal title :
Avicenna Journal of Phytomedicine AJP)
Serial Year :
2016
Journal title :
Avicenna Journal of Phytomedicine AJP)
Record number :
2396398
Link To Document :
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