Author/Authors :
Banava، Sepideh نويسنده Department of Dental Materials,Azad University, Dental Branch,Tehran,Iran , , Najibfard، Kaveh نويسنده San Antonio,USA , , Garcia-Godoy، Franklin نويسنده Bioscience Research Center, College of Dentistry,University of Tennessee Health Science Center,Memphis,USA , , Saghiri، Mohammad Ali نويسنده School of Medicine and Public health,Department of Ophthalmology & Visual Sciences,University of Wisconsin,Madison,USA , , Ghahremani، Mohammad Hossein نويسنده Faculty of Pharmacy, Pharmaceutical Sciences Research Centre,Department of Toxicology and Pharmacology,Tehran University of Medical Sciences,Tehran,Iran , , Ostad، Naser نويسنده Faculty of Pharmacy, and Pharmaceutical Sciences Research Centre,Department of Toxicology and Pharmacology,Tehran University of Medical Sciences,Tehran,Iran ,
Abstract :
Background and aims. The aim of this study was to evaluate the effect of dilution and curing methods of an etch and rinse adhesive and a self etching primer from the same manufacturer at early exposure time on cytotoxicity of primary human gingival fibroblasts.Materials and methods. Primary human gingival fibroblasts were exposed to different dilutions of Adper Single Bond (ASB) and Adper Prompt L Pop (APL) (3M ESPE, USA). They were evaluated in unpolymerized mode for 20 s, 5 min and 24 h and in polymerized mode for 24 h and 48 h. Cytotoxicity was evaluated using three cytotoxic tests (MTT, cell counting and DNA condensation). Data was analyzed by a one way ANOVA and Post Hoc Tukey HSD test.Results. Cytotoxicity tests revealed that unpolymerized APL was more cytotoxic compared to ASB after 20 s (P<0.05). By increasing the time to 5 min and 24 h, ASB was more cytotoxic than APL with lower dilutions. Polymerized ASB was more toxic than APL.Conclusion. Both adhesives were cytotoxic in different dilutions, times and curing modes. Cytotoxicity of the unpolymerized self etching primer (APL) was more than etch and rinse adhesive (ASB) in 20 s, which is important clinically and dentists should be aware of the harmful effects and try to minimize it by curing and rinsing soon after composite resin insertion. ASB was more cytotoxic at 5 min and 24h.
