Author/Authors :
Karimfar ، Mohammad Hassan نويسنده Department of Anatomy, School of Medicine, Ilam University of Medical Sciences, Ilam- Iran. , , Noorozian، Mohsen نويسنده Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Noorozian, Mohsen , Mastery Farahani، Reza نويسنده Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Mastery Farahani, Reza , Sadat Khoramgah، Maryam نويسنده Department of Medical Biotechnology, School of Medicine, Shahid Beheshti University of Medical Sciences and Health Services, Tehran, Iran. Sadat Khoramgah, Maryam , Azimi، Hadi نويسنده PhD student , , Keramatinia، Ali Asghar نويسنده Cancer Research Center, Shahid Beheshti University of Medical Sciences and Health Services, Tehran, Iran. Keramatinia, Ali Asghar , Ahmadi Rozbahani، Navid نويسنده Hearing Disorders Research Center, Shahid Beheshti University of Medical Sciences and Health Services, Tehran, Iran. Ahmadi Rozbahani, Navid , Bahadori Monfared، Ayad نويسنده Department of Epidemiology, Faculty of Health, Shahid Beheshti University of Medical Sciences, P.O. Box 19395-4741, Tehran, I.R. Iran , , Abbaszadeh، Hojjat-Allah نويسنده Department of Health & Community Medicine, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Abbaszadeh, Hojjat-Allah
Abstract :
Introduction: Multiple sclerosis (MS) is a disease of the immune system: it attacks the myelin around the axons and leaves them exposed. Destruction of myelin weakens the electrical conduction of ions and thus leads to a lack of communication in the nervous system.
Methods: In the present study, we constructed recombinant plasmid and then transformed to E. coli cell. The colonies containing plasmid were selected by Colony PCR. Enzyme digestion and sequencing were utilized to approve the accuracy of the extracted plasmid of these clones. Recombinant plasmid transfect in to mesanchymal stem cells.
Results: Plasmid was verified correctly. After transfection, the transcription of MOG gene and the expression of MOG protein were proved by RT-PCR, western blotting and Elisa.
Conclusion: Plasmid was constructed correctly and mesenchyme stem cells were successfully transfected by transfection and protein can be expressed well, setting a proper foundation for the future studies on the transplantation of gene modified mesanchymal stem cells in order to promote Multiple sclerosis.
