Author/Authors :
Mehri، Soghra نويسنده , , Dadesh، Qutaiba نويسنده School of Pharmacy, Mashhad University of Medical
Sciences, Mashhad, IR Iran , , Tabeshpour، Jamshid نويسنده School of Pharmacy, Mashhad University of Medical
Sciences, Mashhad, IR Iran , , Vahdati Hassani، Faezeh نويسنده School of Pharmacy, Mashhad University of Medical
Sciences, Mashhad, IR Iran , , Karimi، Gholamreza نويسنده , , Hosseinzadeh، Hossein نويسنده ,
Abstract :
Neurotoxicity is one of the most recognized effects of acrylamide
(ACR) in humans and animals. Oxidative stress is an important mechanism
in ACR-induced neurotoxicity. In this research, the effect of silymarin
as a potent antioxidant was evaluated against ACR-induced toxicity in
both in vitro and in vivo models. For the in vitro assay, PC12 cells
were exposed to different concentrations (2.5 - 100 µM) of silymarin for
24 hours. ACR at a final concentration of 5 mM was added and cell
viability was determined using the MTT assay. For the in vivo study,
neurotoxicity was induced using intraperitoneal (IP) administration of
ACR (50 mg/kg) for 11 days. The effects of different doses of silymarin
(40, 80, and 160 mg/kg IP, respectively) were evaluated in ACR-induced
neurotoxicity in Wistar rats based on gait scores. Exposure to ACR
reduced cell viability in PC12 cells. Pre-treatment of cells with
silymarin (100 µM) significantly reduced ACR-induced toxicity. In
addition, administration of ACR induced severe toxicity in Wistar rats,
while silymarin at a dose of 160 mg/kg could improve the rats’ gait
abnormalities. With regard to the potent antioxidant properties of
silymarin, the neuroprotective effects of this natural compound
suggested in the current study may in part be mediated through
inhibition of oxidative stress.
