Synthesis of Hydroxyapatite/Ag/TiO2 Nanotubes and Evaluation of Their Anticancer Activity on Breast Cancer Cell Line MCF-7

In this research, TiO 2 nanotubes were synthesized by anodized oxidation method and were covered with a hydroxyapatitesilver nanoparticles using photodeposition and dip coating for loading silver nanoparticles and coated hydroxyapatite (HA). The morphological texture of TiO 2 nanotube and AgHA nanoparticles on TiO 2 nanotubes surface were studied by field emission scanning electron microscopy (FESEM), energy dispersive Xray spectroscopy (EDAX analysis) and Xray diffraction (XRD). The MCF7 cell lines were treated with concentrations 1, 10 and 100 µg/ml of TiO 2 nanotubes and HA/Ag/TiO 2 nanotube for 24 and 48h. Finally, the cell viability and IC50% were evaluated using MTT assay. The results show that the HA/Ag/TiO 2 has more positive effect on enhancing the cell death compare to TiO 2 nanotubes and also exerts a time and concentrationdependent inhibition effect on viability of MCF7 cells