Author/Authors :
Khosravy، Mohammad Sadeq نويسنده Production and research Complex,Department of Hepatitis and AIDS, Department of Laboratory Animal Sciences,Pasteur Institute of Iran,Tehran,Iran , , Shafiee Ardestani، Mehdi نويسنده Faculty of Pharmacy,Department of Radiopharmacy,Tehran University of Medical Sciences,Tehran,Iran , , Ahangari Cohan، Reza نويسنده Department of Pilot Biotechnology,Pasteur Institute of Iran,Tehran,Iran , , Doroud، Delaram نويسنده Production and research Complex,Department of Quality Assurance,Pasteur Institute of Iran,Tehran,Iran , , Amini، Safieh نويسنده Department of Hepatitis and AID,Pasteur Institute of Iran,Tehran,Iran , , Momen، Seyed Bahman نويسنده Department of Pilot Biotechnology,Pasteur Institute of Iran,Tehran,Iran , , Atyabi، Seyed Mohammad نويسنده Department of Pilot Biotechnology,Pasteur Institute of Iran,Tehran,Iran , , Heydari، Hossien نويسنده Production and research Complex,Department of Quality Assurance,Pasteur Institute of Iran,Tehran,Iran , , Vahabpour، Rohollah نويسنده Department of Hepatitis and AIDS,Pasteur Institute of Iran,Tehran,Iran ,
Abstract :
Manufacturing new Hepatitis B virus vaccines, specifically by the use of nanoparticles, is of high global interest. In this paper, a new biocompatible and biodegradable structure of nano sized hepatitis B virus’ surface antigen (HBsAg) was generated by conjugation with dendrimers, a low cost biodegradable and biocompatible polymer. The physicochemical properties of the conjugate were characterized by zeta potential and size distribution analyses and FT IR spectrometry. The results confirmed the conjugation between HBsAg and the dendrimers. Immunological assays indicated that the immunogenicity of the conjugated HBsAg is more than HBsAg alone. Further investigations are required to explore the mechanisms of action and the exact immunological pathway(s) of this nanocomplex vaccine candidate.
