Author/Authors :
Paiva Costa، Lincoln نويسنده Hospital Madre Teresa, Belo Horizonte,
Brazil , , Moreira Teixeira، Luiz Eduardo نويسنده Hospital Madre Teresa and Universidade Federal de Minas
Gerais Minas Gerais, Belo Horizonte, Brazil , , Maranhão Lima، Gustavo Silame نويسنده Hospital das Cl?nicas da Universidade Federal de Minas
Gerais, Belo Horizonte, Brazil , , Mendes Ferreira، Marcelo نويسنده Hospital Madre Teresa, Belo Horizonte,
Brazil , , de Andrade، Mateus Antunes نويسنده Hospital Madre Teresa, Belo Horizonte,
Brazil , , Teixeira Vidigal، Paula Vieira نويسنده Universidade Federal de Minas Gerais Minas Gerais, Belo
Horizonte, Brazil , , Gomes Faraco، André Augusto نويسنده Universidade Federal de Minas Gerais Minas Gerais, Belo
Horizonte, Brazil , , Temponi، Eduardo Frois نويسنده Hospital Madre Teresa, Belo Horizonte,
Brazil , , de Araújo، Ivana Duval نويسنده Universidade Federal de Minas Gerais Minas Gerais, Belo
Horizonte, Brazil ,
Abstract :
The systemic antimicrobial prophylaxis is the standard treatment in
the prevention of osteomyelitis after open fractures, with topical
application of antimicrobials as an alternative due to their high
concentrations at the site of the fracture, low systemic concentrations
and fewer side effects. This study aimed to evaluate the effectiveness
of prophylaxis of osteomyelitis through experimental model of open
fractures with the use of chitosan films, whether or not impregnated
with ciprofloxacin. In this experimental study, 24 Holtzman rats were
distributed into 4 groups of 6 rats each. The CT (control of treatment)
group: an open fracture model treated with systemic antimicrobial; the
IC (infection control) group: an open fracture untreated model; the C
(chitosan) group: an open fracture model treated using a chitosan film;
and the CA (chitosan with antimicrobial) group: an open fracture model
treated using a chitosan film impregnated with antimicrobial. After 3
weeks the animals were killed by an overdose of anesthetic, and a
fragment osseous was removed for histological and microbiological
analysis. The comparisons between the groups considered significant
values of P ≤ 0.05. In cultures of the CT group, there was less
bacterial growth compared to the results of the cultures of the IC (P =
0.005), C (P = 0.005) and CA (P = 0.009) groups. The inflammation was
lower in the CT group compared to the IC (P = 0.014), C (P = 0.001) and
CA (P = 0.007) groups. In this experimental model of open fracture, the
chitosan film pure or impregnated with ciprofloxacin was not effective
in the prophylaxis of osteomyelitis.
