Author/Authors :
Dabiri، Shahriar نويسنده Pathology and Stem cell research center, Afzalipour Medical School,Pathology Department,Kerman University of
Medical Science,Kerman,Iran , , Moeini aghtaei، Mohammadmehdi نويسنده Pathology and Stem cell research center, Afzalipour Medical School,Pathology Department,Kerman University of
Medical Science,Kerman,Iran , , Shahryari، Jahanbano نويسنده Pathology and Stem cell research center, Afzalipour Medical School,Pathology Department,Kerman University of
Medical Science,Kerman,Iran , , Shamis meymandi، Manzume نويسنده School of Medicine,Dept. of Pharmacology,Kerman University of Medical Sciences,Kerman,Iran , , Amirpour-Rostami، Sahar نويسنده Pharmaceutics Research Center, Institute of Neuropharmacology,Kerman University of Medical Sciences,Kerman,Iran , , Foutohi-Ardekani، Reza نويسنده Molecular and Medicine Research Center,Arak University of Medical Sciences,Arak,Iran ,
Abstract :
Background: The breast cancer is the most prevalent cancer among women, on the other hand absence of myoepithelial cells play a pivotal role in pathogenesis of this cancer. Thus we aimed to investigate the possible abilities of the molecular assay technique to find a relationship between mammary serine protease inhibitor (Maspin) gene expression possibly secreted by myoepithelial cells, grade of breast cancer and other prognostics factors (ER, PR, and cerbB2). Methods: Paraffin embedded blocks of 31 breast cancer patients together with two normal breast tissues were used for IHC staining and Maspin gene RNA detection uses the realtime PCR method. Applying QIAGEN kit, we were able to measure Maspin RNA and Extract the cDNA of different sles for evaluating the Maspin RNA level. Results: We found that the RNA level was considerably lowerin these cancer sles compared with normal sles. In addition, different grades of breast cancer in the obtained results adopt some distinguishable values. The Maspin expression in sles with grades II and III is much lower than the ones in normal group (Plt 0.05) which could be considered as a promising way in diagnosing of this disease. The results showed no considerable differences in Maspin gene expression of the cerbB2 scores in the tumor group except the sles having score 0. The other observation of this research study confirmed that Maspin gene expression couldn#039 t show any differences between the values of both ER and PR in different scores of the tumor group. On the other hand, the cDNA of these patients showed lower values compared with normal sles. Conclusion: Maspin expression was reduced in sles with grade II III of invasive ductal carcinoma. Based on expression of Maspin IncerbB2, it seems that more expression happened in normal group comparing with different scores of it. We could suggest that there was a reverse relationship between tumor formation and Maspin gene expression. These results showed possible role of Maspin as prognostic factor.
Keywords :
Real-Time PCR , Iran , breast cancer , Prognostic factor , Maspin gene
