Comparing Rapid and Specific Detection of Brucella in Clinical Samples by PCR-ELISA and Multiplex-PCR Method

Background: Rapid diagnosis and differentiation of Brucella is of high importance due to the side effects of antibiotics for the treatment of brucellosis. This study aimed to identify and compare PCRELISA as a more accurate diagnositc test with other common molecular and serological tests. Methods: In this experimental and sectional study, during March 2014 to Sep 2015, 52 blood sles of suspected patients with clinical symptoms of brucellosis were evaluated in medical centers all over Iran with serum titers higher than 1:80. Using two pairs of specific primers of Brucella abortus, B. melitensis and DIGdUTP, Fragment IS711 (The common gene fragment in B. melitensis and B. abortus) was lified. DIGELISA was performed using specific probes of these 2 species of Brucella and patterns were subsequently analyzed, then positive responses were compared by detecting gel electrophoresis. Results: PCRELISA method detected all 28 sles from 52 positive sles. Its sensitivity was 6.0 pg concentration of genomic DNA of Brucella. In gel electrophoresis method, 22 sles of all positive sles were detected. PCRELISA was more efficient than PCR and bacterial culture method at Pvalue Conclusion: PCRELISA molecular method is more sensitive than other molecular methods, lack of mutagenic color and also a semiquantitative ability. This method is more effective and more accurate compared to PCR, serology and culture of bacteria. PCRELISA does not have false responses. The limitation of this method is detection of bacteria in the genus compared to Multiplex PCR and Gel Electrophoresis.