Effect of dietary Satureja khuzistanica powder on semen characteristics and thiobarbituric acid reactive substances concentration in testicular tissue of Iranian native breeder rooster

36 قطعه خروس بومي اصفهان در سن 40 هفتگي به‌ طور تصادفي درون قفس‌هاي انفرادي قرار گرفتند و به سه گروه 12 تايي (هر گروه با سه تكرار) تقسيم شدند. گروه شاهد روزانه 100 گرم جيره پايه دريافت كرد و به جيره ساير تيمارها به ترتيب سطوح 20 و 40 گرم در كيلوگرم پودر گياه مرزه خوزستاني (تيمار دو و سه) به مدت 8 هفته افزوده شد. در پايان هر هفته ويژگي‌هاي مني مورد بررسي قرار گرفتند. در پايان هفته‌ هشتم خروس‌ها كشتار شدند و غلظت TBARS در بافت بيضه 18 خروس مورد ارزيابي قرار گرفت. غلظت TBARS بافت بيضه تحت تأثير تيمارهاي آزمايشي قرار نگرفت (P>0.05). درصد ناهنجاري اسپرم در تيمارهاي آزمايشي كمتر از گروه شاهد بود (P<0.05). درصد جنبايي اسپرم در تيمار سه بالاتر از ساير گروه‌ها و در تيمار دو بالاتر از گروه شاهد بود (P<0.05). غلظت اسپرم تحت تاثير سطوح جيره‌اي پودر گياه مرزه خوزستاني قرار نگرفت (P>0.05).

Because of a paucity of information on the effect of Satureja khuzistanica in male chickens, this study was undertaken to determine the influence of dietary S. khuzistanica powder (SKP) on seminal characteristics and testes thiobarbituric acid reactive substances (TBARS) content in Iranian native breeder rooster. Thirty six 40 week old roosters were randomly allotted to 3 equal groups and received either a basal diet without SKP (T1 or control), or a diet containing 20 g/kg (T2) and 40 g/kg (T3) of SKP for 8 week long experimental period. Semen sles were obtained weekly by abdominal massage to evaluate the seminal characteristics. At the end of the eighth week 18 birds (6 birds per each group) were randomly slaughtered, and sle was taken from right testes for TBARS evaluation. Administration of SKP improved all semen traits, except for sperm concentration. Likewise, TBARS content in SKP treatments did not significantly differ from the control (P>0.05). Seminal volume, live sperm percentage and plasma membrane integrity percentage in SKP treated groups were higher than the control. Conversely, abnormal sperm percentages reduced in SKP treated groups (P<0.05). Plasma membrane integrity in experimental treatments was significantly higher than the control in 2nd, 3rd and 7th weeks. However, at 6th and 8th weeks only T3 treatment was significantly different from the control. Notably, there was an increase in total sperm concentration in SKP treated groups in compared to the control birds. In conclusion, this studyindicated that addition of SKP in rooster diet improves sperm quality and also reduces their sperm membrane lipid peroxidation, which may lead to higher fertilization rate.