Author/Authors :
Mohammadi، Javad نويسنده , , Mohammadi، Mehdi نويسنده Eslamabad-E-Gharb branch, Islamic Azad University, Eslamabad-E-Gharb, Iran Mohammadi, Mehdi , Mehrasbi، Mohammad Reza نويسنده Department of Environmental Health, Zanjan University of Medical Sciences, Zanjan, Iran. Mehrasbi, Mohammad Reza , Peyda، Mazyar نويسنده Department of Environmental Health Engineering, Zanjan University of Medical Sciences, Zanjan, Iran Peyda, Mazyar
Abstract :
Background; Enzymes are well known as sensitive catalysts in the laboratory and industrial scale. To improve their properties and for using their significant potential in various reactions as a useful catalyst the stability of enzymes can often require improvement. Enzymes Immobilization on solid supports such as epoxy- functionalized ferric silica nanocomposite can be effective way to improve their characteristics.
Methods; In this study silica coated magnetite nanoparticles were Functionalized with GPTSM as a linker, then immobilization reaction performed by using various amounts of lipase B from Candida Antarctica (CALB), for the next step immobilization effects on thermal stability and optimum pH were investigated in comparison with free CALB.
Results; Results illustrated enzyme was successfully immobilized on nano particles and immobilized derivative retains 100% of its activity by 55°C while free CALB loss its activity at the same condition.
Conclusion; Immobilization of CALB on Fe3O4@SiO2 particles resulted in significant improvements in its characteristics such as thermal stability and methanol tolerance compared to the free CALB.
