Author/Authors :
Yang، Weihua نويسنده Medical Research & Laboratory Diagnostic Center, Jinan
Central Hospital, Shandong University, Jinan, 250013,
P.R.China , , Cao، Cuiming نويسنده Medical Research & Laboratory Diagnostic Center, Jinan
Central Hospital, Shandong University, Jinan, 250013,
P.R.China , , Wang، Weichen نويسنده Medical Research & Laboratory Diagnostic Center, Jinan
Central Hospital, Shandong University, Jinan, 250013,
P.R.China , , Wang، Yunshan نويسنده Medical Research & Laboratory Diagnostic Center, Jinan
Central Hospital, Shandong University, Jinan, 250013,
P.R.China ,
Abstract :
Streptococcus agalactiae colonization in pregnant women leads to
prenatal and neonatal infections worldwide; thus, early detection is
very crucial. Development of a rapid detection method for S. agalactiae.
Genomic DNA of cultured S. agalactiae was prepared and loop-mediated
isothermal amplification (LAMP) primers were designed based on the cAMP
gene in bacteria. The optimum primer set was selected based on the
reaction speed and specificity. The reaction result was monitored
visually. The sensitivity and specificity of the LAMP method were
evaluated and compared with polymerase chain reaction (PCR) method.
Using the optimum primer set the reaction can be completed in 40 minutes
at 63°C in a water bath. The LAMP assay was 10 - 100 times more
sensitive than PCR, with a detection limit of 10 CFU/mL of S.
agalactiae. Forty vaginal swab were examined by LAMP, and the
specificity was 100%. Thus, for S. agalactiae detection, the LAMP method
is a valuable diagnostic tool, with easy, rapid, visual, accurate, and
sensitive advantages.
