Author/Authors :
Hua، Xiaoting نويسنده Department of Infectious Diseases, Sir Run Run Shaw
Hospital, College of Medicine, Zhejiang University, Hangzhou,
Zhejiang 310016, China , , Qu، Tingting نويسنده State Key Laboratory for Diagnosis and Treatment of
Infectious Disease, First Affiliated Hospital, College of Medicine,
Zhejiang University, Hangzhou, Zhejiang 310003, China , , Li، Xi نويسنده , , Chen، Qiong نويسنده Department of Infectious Diseases, Sir Run Run Shaw
Hospital, College of Medicine, Zhejiang University, Hangzhou,
Zhejiang 310016, China , , Ruan، Zhi نويسنده Department of Infectious Diseases, Sir Run Run Shaw
Hospital, College of Medicine, Zhejiang University, Hangzhou,
Zhejiang 310016, China , , Yu، Yunsong نويسنده Department of Infectious Diseases, Sir Run Run Shaw
Hospital, College of Medicine, Zhejiang University, Hangzhou,
Zhejiang 310016, China ,
Abstract :
Enterococcus faecium is an important nosocomial pathogen and has
developed resistance to multiple antibiotics, such as vancomycin.
Nitrofurantoin is active against E. faecium and E. faecalis, including
vanA- and vanB-positive isolates. To investigate the role of rpoB
mutation in E. faecium. The growth rate and reactive oxygen species
(ROS) levels were determined. The proteomic profiles of E. faecium B42
and its Rifr mutant B42-R7 were also compared. Complement experiments
were performed to elucidate the role of nitroreductase in nitrofurantoin
resistance. The Rifr mutant (B42-R7, rpoB H489D) of E. faecium had a
lower growth rate and higher ROS levels. Overexpression of
nitroreductase in E. faecium B42-R7 was observed in proteomic analyses.
Enhanced expression of nitroreductase in Escherichia coli increased the
sensitivity to nitrofurantoin. The rpoB mutation in E. faecium not only
altered the susceptibility to rifampin, but also affected global protein
expression, including nitroreductase. Nitroreductase expression in E.
faecium B42-R7 might play a role in nitrofurantoin resistance.
