Author/Authors :
Mukhtar، Nadia نويسنده Department of Microbiology, University of Veterinary and
Animal Sciences, Lahore, Pakistan , , Yaqub، Tahir نويسنده Department of Microbiology, University of Veterinary and
Animal Sciences, Lahore, Pakistan , , Masood، Ambreen نويسنده Department of Microbiology, University of Veterinary and
Animal Sciences, Lahore, Pakistan , , Javed، Hasnain نويسنده Department of Microbiology and Molecular Genetics, Quaid-e-Azam Campus, University of the Punjab, Lahore-54590, Pakistan , , Nazir، Jawad نويسنده Department of Microbiology, University of Veterinary and
Animal Sciences, Lahore, Pakistan , , Aslam، Asim نويسنده Department of Microbiology, University of Veterinary and
Animal Sciences, Lahore, Pakistan , , Ali، Akhtar نويسنده , , Javed، Maryam نويسنده Department of Microbiology, University of Veterinary and
Animal Sciences, Lahore, Pakistan , , Nadeem، Asif نويسنده Department of Microbiology, University of Veterinary and
Animal Sciences, Lahore, Pakistan , , Hussain، Tanveer نويسنده Department of Molecular Biology, Virtual University of
Pakistan, Lahore, Pakistan , , Tahir، Zarfashan نويسنده Department of Microbiology, University of Veterinary and
Animal Sciences, Lahore, Pakistan , , Bin Aslam، Hassaan نويسنده Department of Microbiology, University of Veterinary and
Animal Sciences, Lahore, Pakistan ,
Abstract :
Group A bovine Rotaviruses (BRV) are one of the main factors of
neonatal calf diarrhea and mortality around the world. The current study
was carried out to assess the genetics of BRV circulating in the
Pakistani livestock and characterize the antigenic genes of Rotavirus
contributing towards calf diarrhea. A total of 200 fecal samples were
collected from diarrheic buffalo and cattle calves from ten districts of
Punjab, Pakistan. Samples were selected on the basis of agro-ecological
zones of the province. Fecal samples were analyzed for the presence of
BRV using Ag-capture ELISA. Positive samples were subjected to PCR for
the amplification of VP4 and VP6 genes and subsequent sequencing.
Phylodynamics was performed to infer genetic clustering and evolutionary
distances of characterized strains of BRV in accordance to the BRV
available publically. In ELISA, a total of 12 calf samples (5 cattle and
7 buffalo), 6%, were found positive. The sequencing and phylogenetic
analysis of both genes showed that Pakistani BRV (BRV/QOL/13) depicted
maximum identity (98%) of the VP4 gene with Indian BRV VP4 gene.
Pakistani BRV VP6 gene (Pakistan/MM85/QOL) showed maximum identity of
98% with Indian BRV A VP6 gene (accession No. JF720873, EF200565).
Bovine rotavirus presents 6% of calf diarrhea in Pakistani cattle and
buffalo samples. The VP4 and VP6 genes show closed relationships with
bovine rotavirus reported from Indian isolates.
