Author/Authors :
Soltani، Narjes نويسنده Physiology-Pharmacology Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. Soltani, Narjes , Ghayoor Karimiani، Ehsan نويسنده , , Farzanehfar، Mohammadreza نويسنده Department of Internal Medicine, Ghaem Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran , , Mashkani، Baratali نويسنده Department of Medical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran , , Jafarian، Amirhossein نويسنده Cancer Molecular Pathology Research Center, Department of Pathology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran , , Ashraf، Hami نويسنده Research and Education Department, Razavi Hospital, Mashhad, Iran , , Akhavan Rezyat، Arash نويسنده Student Research Committee, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran , , Soukhtanloo، Mohammad نويسنده Department of Biochemistry, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IR Iran ,
Abstract :
Background: Colorectal cancer, the third most common type of cancer is a major
cause of mortality worldwide. If colorectal cancer is detected at the early stages, the
5-year survival rate is 90%. MEIS1 homeobox gene is implicated in numerous solid
tumors and hematological malignancies. Therefore, the present study aims to investigate
the methylation status of the MEIS1 gene in colorectal cancer.
Methods: We used real-time quantitative methylation-specific PCR to detect
MEIS1 promoter methylation in 42 colorectal cancer tissues and 42 normal colorectal
tissues.
Results: Methylation was observed only in the positive control samples - CG
Genome Universal Unmethylated DNA and CG Genome Universal Methylated DNA.
There was no change observed in MEIS1 promoter methylation status in 42 patients.
Conclusion: The results of the current study indicated that the MEIS1 gene
promoter was not methylated in the cases. Gene expression study confirmed the
unmethylated status of the MEIS1 gene in the colorectal cancer process among the studied
population.
