Efficacy of Pistacia khinjuk Fruits on Viability of Hydatid Cyst Protoscoleces and Its Acute Toxicity in Mice Model
MAHMOUDVAND، Hossein نويسنده Research Center for Tropical and Infectious Diseases, Kerman University of Medical Science, Kerman, Iran MAHMOUDVAND, Hossein , MIRBADIE، Seyed Reza نويسنده School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran MIRBADIE, Seyed Reza , GHASEMI KIA، Mehdi نويسنده Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University, Kerman, Iran GHASEMI KIA, Mehdi , Badparva، Ebrahim نويسنده Department of Parasitology and Mycology, Lorestan University of Medical Sciences, Khorramabad, IR Iran Badparva, Ebrahim , SHAMSADINI LORI، Saeedeh نويسنده Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran SHAMSADINI LORI, Saeedeh , Fasihi Harandi، Majid نويسنده Dept. of Medical Parasitology and Mycology, Kerman University of Medical Sciences, Kerman, Iran. Fasihi Harandi, Majid
Background: This investigation aimed to evaluate the in vitro scolicidal effects of Pistacia khinjuk methanolic extract against protoscoleces of hydatid cysts and its acute toxicity in mice NMRI model.
Methods: Protoscoleces were aseptically extracted from sheep livers having hydatid cysts. Various concentrations of the essential oil (12.5- 100 mg/mL) were used for 10 to 60 min. Viability of protoscoleces was confirmed using eosin exclusion test (0.1% eosin staining). Twenty-four male NMRI mice were used to assess the acute toxicity of P. khinjuk.
Results: P. khinjuk extract at the concentrations of 100 mg/mL after 10 min of exposure killed 100% of protoscoleces. Similarly, the mean of mortality rate of protoscoleces after 20 min of exposure to the concentration of 50 mg/mL was 100%. The LD50 of the intraperitoneal injection of the P. khinjuk methanolic extract was 2.8 g/kg and the maximum non-fatal dose was 1.7 g/kg.
Conclusion: The findings demonstrated effective scolicidal effects of P. khinjuk extract with no considerable toxicity that might be a natural source for the producing of new scolicidal agent.