Author/Authors :
Mehrabi Sisakht، Jafar نويسنده Student Research Committee, Shiraz University of Medical
Sciences, Shiraz, IR Iran , , Khosravi، Mohammad Bagher نويسنده Cardiovascular Pharmacology Research Center, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; , , Milani، Soheila نويسنده Anesthesiology and Critical Care Research Center, Shiraz
University of Medical Sciences, Shiraz, IR Iran , , Kazemi، Masoumeh نويسنده Departments of Medical Genetics, Shiraz University of
Medical Sciences, Shiraz, IR Iran , , Tabei، Seyed Mohammad Bagher نويسنده Departments of Medical Genetics, Shiraz University of
Medical Sciences, Shiraz, IR Iran ,
Abstract :
Inflammatory events following brain death (BD) decrease the quality
of donor organs which can affect the outcome of the transplantation. It
experimentally and clinically is verified that the level of inflammatory
cytokines is increased in the BD of the donor results. In experiments,
the in vitro use of vitamin C can successfully decrease the levels of
inflammatory cytokines. The aim of this study was to investigate the
influence of vitamin C administration on the inflammatory status of the
graft in BD liver donors. This study was conducted at Nemazee Hospital’s
transplant center (Shiraz, Iran). In this interventional study, the BD
liver donors (n = 40) were randomly divided into two groups. The control
group received only the routine intensive care unit (ICU)
considerations, and the intervention group was treated with Vitamin C
before harvesting organ (100 mg/kg, initially followed by 100 mg/kg/6h
until organ removal). Blood samples were taken from BD patients in the
intervention and control groups 3 times: 6 hours before operation,
immediately after laparotomy, and immediately prior to clamping the
aorta to assess the gene expression ratio of Interleukin 1 beta (IL1B)
and Interleukin 10 (IL10) cytokines using real time polymerase chain
reaction (PCR) (n = 40). Soluble serum cytokines were measured by
enzyme-linked immunosorbent assay (ELISA) (n = 24). No significant
differences were observed in mRNA expression ratio of IL1B and IL10
between two groups. Despite an acceptable decrease in serum
concentration of the inflammatory markers IL1B and IL10 at time point 2
minus time point 1 (T2-1), no significant differences were observed in
vitamin C-treated BD donors compared with the control donors. There was
a significant difference among aspartate aminotransferase (AST) and
alanine aminotransferase (ALT) changes on the 3rd to 1st days after
operation between the control and intervention recipients (P <
0.05). The present study suggests a beneficial effect of vitamin C
adsministration on post-transplant function of the liver from BD liver
donors.
