Author/Authors :
Mehrabani، Mehrnaz نويسنده Department of Pharmacology, Iran University of Medical
Sciences, Tehran, IR Iran , , Seyyedkazemi، Seyyed Mohsen نويسنده Herbal and Traditional Medicines Research Center, Kerman
University of Medical Sciences, Kerman, IR Iran , , Nematollahi، Mohammad Hadi نويسنده Research Center for Hydatid Disease in Iran, Kerman
University of Medical Sciences, Kerman, IR Iran , , Jafari، Elham نويسنده Tehran University of Medical Sciences , , Mehrabani، Mitra نويسنده , , Mehdipour، Mohammad نويسنده Department of Pharmacology and Toxicology, School of
Pharmacy, Kerman University of Medical Sciences, Kerman, IR
Iran , , Sheikhshoaee، Zahra نويسنده Department of Pathology, Kerman University of Medical
Sciences, Kerman, IR Iran , , Mandegary، Ali نويسنده ,
Abstract :
A combination of the oils of sesame, hemp, wild pistachio, and
walnut has been used for treatment of skin disorders, including wound
burns, in some parts of Kerman, Iran. Evaluation of this remedy in the
form of a pharmaceutical formulation in animal models can pave the way
for its future application in wound burn healing in humans. This
experimental study investigated the healing potential of a new formula
(NF) based on folk medicine from Iran for the treatment of third degree
burns in mice. The formula was a combination of the oils of four plants:
sesame (Sesamum indicum L.), wild pistachio (Pistacia atlantica Desf.),
hemp (Cannabis sativa L.), and walnut (Juglans regia L.) Twenty-four
mice were selected based on simple random sampling. Twenty-five percent
of the total body surface area was burned by exposure to boiling water,
according to the Walker-Mason method. NF and silver sulfadiazine (the
positive control) were applied topically twice a day for 21 days. The
burned area in the negative control group was left untreated.
Epithelialization time and the percent of wound contraction were
measured during the treatment period. The process of wound repairing was
evaluated using histological (H and E and trichrome staining) and
immunohistological (anti-pancytokeratin) methods. When compared to the
controls, NF significantly improved wound contraction after day 10.
Epithelialization time in the NF group was significantly faster than in
the other groups (20 vs. 25.5 days) (P < 0.001).
Histopathological and immunohistochemical findings confirmed the
efficacy of the NF. A new therapeutic remedy was introduced for the
treatment of burn wounds. Further clinical and molecular studies are
suggested to determine the exact mechanism(s) involved in the burn wound
healing effect of NF.
