Author/Authors :
Caglayan Sozmen، Sule نويسنده Department of Pediatrics, Division of Pediatric Allergy and Immunology, Faculty of Medicine, Dokuz Eylul University Izmir,Turkey Caglayan Sozmen, Sule , Karaman، Meral نويسنده Department of Medical Microbiology, Faculty of Medicine, Dokuz Eylul University Izmir, Turkey Karaman, Meral , Cilaker Micili، Serap نويسنده Department of Histology and Embryology, Faculty of Medicine, Dokuz Eylul University Izmir, Turkey Cilaker Micili, Serap , Isik، Sakine نويسنده Department of Pediatrics, Division of Pediatric Allergy and Immunology, Faculty of Medicine, Dokuz Eylul University Izmir,Turkey Isik, Sakine , Bagriyanik، H Alper نويسنده Department of Histology and Embriology, Dokuz Eylul University Hospital, Balcova, Izmir, Turkey Bagriyanik, H Alper , Arikan Ayyildiz، Zeynep نويسنده Department of Pediatrics, Division of Pediatric Allergy and Immunology, Faculty of Medicine, Dokuz Eylul University Izmir,Turkey Arikan Ayyildiz, Zeynep , Uzuner، Nevin نويسنده Department of Pediatrics, Dokuz Eylul University Hospital, Division of Allergy, Balcova, Izmir, Turkey Uzuner, Nevin , Anal، Ozden نويسنده Department of Pediatrics, Division of Pediatric Allergy and Immunology, Faculty of Medicine, Dokuz Eylul University Izmir,Turkey Anal, Ozden , Karaman، Ozkan نويسنده Department of Pediatrics, Dokuz Eylul University Hospital, Division of Allergy, Balcova, Izmir, Turkey Karaman, Ozkan
Abstract :
Quercetin is a dietary flavonoid which has anti-inflammatory effects. This study aimed to evaluate the influence of quercetin on histopathological aspects and airway epithelium in allergic airway inflammation mice model. Twenty-eight BALB/c mice were randomly divided into four groups: Group I (control), Group II (untreated mice with allergic airway inflammation), Group III (allergic airway inflammation quercetin-treated [16mg/kg/day]), Group IV (allergic airway inflammation dexamethasone-treated [1mg/kg/day]). Ovalbumin was administered intraperitoneally and via inhalation to achieve allergic airway inflammation mice model and treatments were also given intraperitoneally. Epithelium thickness, subepithelial smooth muscle thickness, number of mast and goblet cells, and basement membrane thickness were examined on samples isolated from lung. Immunohistochemical evaluationof lung tissues was performed using IL-25, IL-33, thymic stromal lymphopoietin (TSLP), terminal deoxynucleotidyl transferase-mediated dUTP nick endlabeling (TUNEL) and cysteine-dependent aspartate-specific proteases(caspase)-3 antibodies. IL-4, IL-25, IL-33, TSLP were quantified in bronchoalveolar lavage (BAL) and OVAspecific IgE levels was measured in serum by standard ELISA protocols. IL-25, IL-33, thymic stromal lymphopoietin (TSLP) and cysteine-dependent aspartate-specific proteases (caspase)-3. Quercetin treatment led to lower epithelial thickness, subepithelial smooth muscle thickness, goblet and mast cell numbers compared to untreated mice with allergic airway inflammation (p<0.05). However, quercetin treatment was not effective on improving basal membane thickness. Immunohistochemical scores of IL-25, IL-33, TSLP, caspase-3 and TUNEL were lower in quercetin-treated mice t compared to untreated mice with allergic airway inflammation (p<0.05). IL-4, IL-25, IL-33, TSLP levels in BAL and OVA-specific IgE in serum were lower in quercetin treated mice compared to untreated mice (p<0.05). These findings suggest that quercetin improves chronic histopathological changes except basal membrane thickness in lung tissue and its beneficial effects on inflammation might be related to modulating epithelium derived cytokines and epithelial apoptosis.
