Discrimination of Paederus fuscipes and Paederus littoralis by mtDNA-COI PCR-RFLP
Bazrafkan، Sahar نويسنده Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Bazrafkan, Sahar , Vatandoost، Hassan نويسنده , , Heydari، Abbas نويسنده , , Bakhshi، Hassan نويسنده Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Bakhshi, Hassan , Panahi-Moghadam، Somayeh نويسنده Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Panahi-Moghadam, Somayeh , Hashemi-Aghdam، Saedeh نويسنده Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Hashemi-Aghdam, Saedeh , Mohtarami، Fatemeh نويسنده Department of Medical Entomology and Vectors Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Mohtarami, Fatemeh , Rahimi-foroushan، Abbas نويسنده Esfahan University of Medical Sciences, Esfahan, Iran Rahimi-foroushan, Abbas , Anlaş، Sinan نويسنده Celal Bayar University Ala?ehir, Vocational School, Department of Entomology, Ala?ehir, Manisa, Turkey Anlaş, Sinan , Shayeghi، Mansoreh نويسنده Department of Entomology, Tehran University of Medical Sciences, Tehran, Iran Shayeghi, Mansoreh , Oshaghi، Mohammad Ali نويسنده Tehran University of Medical Sciences, Tehran, Iran. , , Abtahi، Seyed Mohammad نويسنده Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Abtahi, Seyed Mohammad
Background: Linear dermatitis is endemic in Iran where most cases occur in the Caspian Sea coast and Fars province. The disease is caused by beetles of the genus Paederus which are active from early spring to beginning of autumn although its incidence rises from May to August. The classic taxonomy of Paederus spp. is based on the male genitalia that is very complex and needs expertise. In this study, we report a DNA-based method to discriminate Paederus fuscipes and Paederus littoralis (=syn: P. lenkoranus, P. ilsae).
Methods: Type specimens were collected from north and south of Iran. Molecular typing of the species was performed using restriction fragment length polymorphism (RFLP) analysis of polymerase chain reaction (PCR)-amplified fragments of mtDNA-COI.
Results: Sequence analyses of the data obtained in this study showed significant DNA polymorphisms. There were 89 substitutions between COI sequences of the two species. The mtDNA-COI fragment comprises several useful species-specific restriction sites comprising HaeIII that could result in distinctively different species-specific PCR–RFLP profiles. The HaeIII enzyme cuts the 872 bp PCR amplicon of P. littoralis into 737 and 100 bp and two small nonvisible bands whereas it does not cut P. fuscipes amplicon into fragments.
Conclusion: This study demonstrates that molecular typing is useful method and allows one to differentiate between two species and is recommended for discrimination of other Paederus species, which morphologically are indistinguishable or very difficult to be distinguished.