Author/Authors :
Naserpour Farivar، Taghi نويسنده , , Peymani، Amir نويسنده Microbiology Department, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran , , Najafipour، Reza نويسنده Cellular and Molecular Research Centre, Qazvin University of Medical Sciences, Qazvin, Iran , , Aslani Mehr، Masoumeh نويسنده Cellular and Molecular Research Center, Qazvin University
of Medical Sciences, Qazvin, IR Iran , , Alizadeh، Safar Ali نويسنده Department of Clinical Biochemistry and Medical Genetics, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran , , Johari، Pouran نويسنده Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, IR Iran ,
Abstract :
Organophosphate pesticides (OP) are applied to agricultural farms
and can be carried away into closely sewerage and gullies, which
consequently carry water to rivers and lakes and when distributed in the
environment they become polluted and require remediation. The current
study aimed at producing a genetically engineered Pseudomonas
plecoglossicida capable of biodegradation of the organophosphate
pesticides, paraoxon. Genetically engineered P. plecoglossicida was
initially made by transferring polymerase chain reaction (PCR) product
of opd gene from Flavobacterium sp. ATCC 27551 into the chromosome of P.
plecoglossicida. The constructed strain could hydrolyze paraoxon to
p-nitrophenol and di-ethylphosphate in paraoxon supplemented in complete
supplement mixture (CSM) medium. The isolate could use paraoxon as the
only source of carbon. Thus, the bacteria degraded the organophosphate
pesticides, and utilized nutrient products of their degradation. The
observed versatility of genetically engineered P. plecoglossicida in
biodegradation of xenobiotics suggested that this strain may be useful
for the multipurpose bioremediation of contaminated agricultural and
industrial sites.
