Author/Authors :
Marashiyan، Seyedeh Mahya نويسنده Department of Basic Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran , , Moradian kouchaksaraei، Fatemeh Fatemeh نويسنده Department of Microbiology Science and Research Branch Islamic Azad University, Guilan, Iran Moradian kouchaksaraei, Fatemeh Fatemeh , Saadatnia، Geita نويسنده Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran , , Golkar، Majid نويسنده ,
Abstract :
Infections by the protozoan parasite Toxoplasma gondii (T. gondii) are widely prevalent in human and animals. SRS3 is a member of SRS antigen family (glycosylphosphatidylinositol-linked proteins), which is structurally related to the highly immunogenic surface antigen SAG1. The SAG family of proteins can cover the surface of T. gondii bradyzoites and tachyzoites. The aim of this study was to evaluate the recombinant SRS3 protein for diagnosis of toxoplasmosis by enzyme-linked immunosorbent assay. Indirect immunoglobulin G (IgG) enzyme-linked immunosorbent assays (ELISAs) with recombinant SRS3 and SAG1 proteins of T. gondii were developed to evaluate the diagnosis of infection in pregnant women. The results showed that sensitivity and specificity were respectively 84.12% and 92% for recombinant protein SRS3 and 92% and 96% for rSAG1. Recombinant proteins SRS3 and rSAG1 are promising antigens in diagnostic assays for detection of specific antibodies against T. gondii.
