Author/Authors :
-، - نويسنده Histology and Embryology group, Basic Science Department, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran|Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran Sahab Negah, Sajad , -، - نويسنده Department of Neuroscience, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran Aligholi, Hadi , -، - نويسنده Histology and Embryology group, Basic Science Department, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran Khaksar, Zabihollah , -، - نويسنده Pediatric Department, Medical Faculty, Shahed University, Tehran, Iran Kazemi, Hadi , -، - نويسنده Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran Modarres Mousavi, Sayed Mostafa , -، - نويسنده Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran|Department of Community Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran Safahani, Maryam , -، - نويسنده Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran Barati Dowom, Parastoo , -، - نويسنده Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran|Epilepsy Research Center, Westf?lische Wilhelms-Universit?t Münster, Germany|Department of Neurology and Department of Neurosurgery, Westf?lische Wilhelms-U Gorji, Ali
Abstract :
Objective(s): In order to grow cells in a three-dimensional (3D) microenvironment, self-assembling peptides, such as PuraMatrix, have emerged with potential to mimic the extracellular matrix. The aim of the present study was to investigate the influence of the self-assembling peptide on the morphology, survival, proliferation rate, migration potential, and differentiation of human meningioma stem-like cells (hMgSCs). Materials and Methods: The efficacy of a novel method for placing hMgSCs in PuraMatrix (the injection approach) was compared to the encapsulation and surface plating methods. In addition, we designed a new method for measurement of migration distance in 3D cultivation of hMgSCs in PuraMatrix. Results: Our results revealed that hMgSCs have the ability to form spheres in stem cell culture condition. These meningioma cells expressed GFAP, CD133, vimentin, and nestin. Using the injection method, a higher proliferation rate of the hMgSCs was observed after seven days of culture. Furthermore, the novel migration assay was able to measure the migration of a single cell alone in 3D environment. Conclusion: The results indicate the injection method as an efficient technique for culturing hMgSCs in PuraMatrix. Furthermore, the novel migration assay enables us to evaluate the migration of hMgSCs.
